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Merck

T9284

Triton X-100

BioXtra

동의어(들):

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS 번호:
UNSPSC Code:
12161900
NACRES:
NA.21
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
2315025
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InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

biological source

synthetic

description

non-ionic

product line

BioXtra

form

liquid

mol wt

micellar avg mol wt 80,000, average mol wt 625

Quality Level

technique(s)

permeability assay: suitable

impurities

≤0.05% Phosphorus (P)

ign. residue

≤0.4%

CMC

0.2-0.9 mM (20-25°C)

transition temp

pour point ~7 °C

solubility

water: soluble

density

1.065 g/cm3 at 20 °C (68 °F)

anion traces

chloride (Cl-): ≤0.05%, sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH4+: ≤0.05%, Na: ≤0.1%, Pb: ≤0.001%, Zn: ≤0.0005%

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General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton X-100 has been used:
  • Along with Span® 80 and Tween® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
  • As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
  • In the blocking solution during immunocytochemistry procedure
  • During deglycosylation procedure
  • For membrane solubilization
  • For cell permeabilization
  • In decellularization of porcine heart valve tissue

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

  • BioXtra Grade product for your Cell Biology and Biochemical research
  • Non-ionic nondenaturing detergent
  • Superior wetting agent and emulsifier
  • Highly versatile surfactant

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Span is a registered trademark of Croda International PLC
TWEEN is a registered trademark of Croda International PLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

저장 등급

10 - Combustible liquids

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Detection of fetal DNA in maternal plasma by microarray coupled with emulsions PCR.
Ge Q, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 369(1), 82-88 (2006)
Structural, metabolic and developmental evaluation of ovulated rabbit oocytes before and after cryopreservation by vitrification and slow freezing.
Theriogenology, 74(5), 847-855 (2010)
Membrane and acto-myosin tension promote clustering of adhesion proteins.
Delanoe-Ayari H, et al.
Proceedings of the National Academy of Sciences of the USA, 101(8), 2229-2234 (2004)
Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea.
Hong JW et al.
Investigative Ophthalmology & Visual Science, 42, 2795-2795 (2001)
Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations.
Dressman D, et al.
Proceedings of the National Academy of Sciences of the USA, 100(15), 8817-8822 (2003)

프로토콜

Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

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