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54457

HEPES

Name: HEPES
Brand: SIGMA

≥99.5% (titration), powder, for molecular biology, BioUltra

동의어(들):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):

C₈H₁₈N₂O₄S

CAS 번호:

7365-45-9

Molecular Weight:

238.30

NACRES:

NA.25

PubChem Substance ID:

57651473

UNSPSC Code:

12161700

EC Number:

230-907-9

MDL number:

MFCD00006158

Beilstein/REAXYS Number:

883043

eCl@ss:

32129211

기술 서비스

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속성

제품 이름

HEPES, BioUltra, Molecular Biology, ≥99.5% (T)

grade

Molecular Biology

Quality Level

200

product line

BioUltra

assay

≥99.5% (T)

form

powder

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

ign. residue

≤0.1% (as SO₄)

loss

≤0.5% loss on drying, 110 °C

pH

5.0-6.5 (25 °C, 1 M in H₂O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

H₂O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤50 mg/kg, sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

1 M in H₂O

UV absorption

λ: 260 nm A_max: 0.045, λ: 280 nm A_max: 0.035

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

설명

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used as a component of:

DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
Recapture solution to re-capture RNA exosomes from the glycerol gradient
ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
2X HBS buffer that is used in transfection process for the production of recombinant lentivectors

Other Notes

3′-Terminal labeling of RNA with T4 RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.

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저장 등급

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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