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Merck

93352

Trizma® base

≥99.0% (T), crystalline, aminopeptidase substrate, BioChemika

동의어(들):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
NH2C(CH2OH)3
CAS 번호:
77-86-1
Molecular Weight:
121.14
NACRES:
NA.25
PubChem Substance ID:
329770229
UNSPSC Code:
12352104
EC Number:
201-064-4
MDL number:
MFCD00004679
Beilstein/REAXYS Number:
741883

주요 문서

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제품 이름

Trizma® base, ≥99.0% (T)

description

aminopeptidase substrate

Quality Level

200

product line

BioChemika

assay

≥99.0% (T)

form

crystalline

loss

≤1% loss on drying, 110 °C

pH

10.5-12.0(4 m in water, 25 °C)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.), 168-172 °C

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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관련 카테고리

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Trizma® base was used as buffer for the following studies:
  • Electrophoretic transfer for the specific identification of isozymes of starch debranching enzyme, α-amylase and 9-amylase.
  • Electrophoretic separation of lipoproteins in polyacrylamide gels.
  • Preparation of TRIS buffer having pH 8.
It may be used to compose DN buffer for DNA nick-end labeling of tissue sections.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

저장 등급

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Cyril Lafon et al.
Ultrasound in medicine & biology, 31(10), 1383-1389 (2005-10-15)
An optically transparent phantom was developed for use in high-intensity focused ultrasound (US), or HIFU, dosimetry studies. The phantom is composed of polyacrylamide hydrogel, embedded with bovine serum albumin (BSA) that becomes optically opaque when denatured. Acoustic and optical properties
Electrophoretic separation of serum lipoproteins in polyacrylamide gel.
C S Frings et al.
Clinical chemistry, 17(2), 111-114 (1971-02-01)
J M Isner et al.
Circulation, 91(11), 2703-2711 (1995-06-01)
Apoptosis has been recognized in normal, including rapidly proliferating, cell populations and is inferred to be potentially responsible for the maintenance of stable cell numbers in tissues with various degrees of proliferative activity. Previous studies performed in rats indicated that
G Kakefuda et al.
Plant physiology, 75(1), 278-280 (1984-05-01)
An electrophoretic transfer technique was developed for the specific identification of isozymes of starch debranching enzyme, alpha-amylase, and beta-amylase. Amylolytic enzymes are separated by native polyacrylamide slab gel electrophoresis and proteins in gels are electrophoretically transferred through starch-containing polyacrylamide gels.
Marcel Kuiper et al.
Biotechnology progress, 35(4), e2821-e2821 (2019-04-16)
Perfusion is a cell culture mode that is gaining popularity for the manufacture of monoclonal antibodies and their derivatives. The cell culture media supporting perfusion culture need to support higher cell densities than those used in fed-batch culture. Therefore, when
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