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Merck

93362

Trizma® base

≥99.8% (T), crystalline, for molecular biology, BioUltra

동의어(들):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
NH2C(CH2OH)3
CAS 번호:
77-86-1
Molecular Weight:
121.14
NACRES:
NA.25
PubChem Substance ID:
57653540
UNSPSC Code:
12352104
EC Number:
201-064-4
MDL number:
MFCD00004679
Beilstein/REAXYS Number:
741883

주요 문서

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제품 이름

Trizma® base, BioUltra, Molecular Biology, ≥99.8% (T)

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

SMILES string

NC(CO)(CO)CO

grade

Molecular Biology

description

aminopeptidase substrate

product line

BioUltra

assay

≥99.8% (T)

form

crystalline

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

ign. residue

≤0.01% (as SO4)

loss

≤0.5% loss on drying, 110 °C

color

colorless to white

pH

10.5-12.0 (25 °C, 4 M in H2O)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)
168-172 °C

solubility

H2O: 4 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤20 mg/kg
sulfate (SO42-): ≤5 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

4 M in H2O

UV absorption

λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.08

suitability

in accordance for luminescence

Quality Level

200

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관련 카테고리

Application

Trizma has been used:
  • In the dissection medium and culture medium of Hippocampal organotypic tissue cultures
  • As a component of lysis buffer before immunoprecipitation and immunoblotting
  • As a component of harvest buffer, lysis buffer, ChIP dilution buffer, and wash buffer in chromatin immunoprecipitation method for studying protein-DNA binding
  • As a component of mixed buffer formulations
  • As a component of NuPAGE loading buffer for SDS-PAGE and as a component of transfer buffer for Western blotting
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma base is used as a general reagent for the preparation of all types of Tris buffers.

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

The BioChemika quality is for the usual biochemical applications. The products designated as BioChemika Ultra grade are suitable for different applications like purification, precipitation, crystallization and other applications which require tight control of elemental content. Trace elemental analyses have been performed for all qualities. The molecular biology qualities are also tested for absence of nucleases and the luminescence quality spectroscopic tests are performed.

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.
Running buffer component in non-denaturing agarose gel electrophoresis; In non-denaturing and urea-denaturing polyacrylamide gel electrophoresis; DNA digest analysis with capillary electrophoresis.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

저장 등급

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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시험 성적서(COA)

Lot/Batch Number
BCCH2012
BCCG1067
BCCF7960
BCCF0089
BCCD3283

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

In vitro Detection of S-acylation on Recombinant Proteins via the Biotin-Switch Technique.
Qi D and Innes RW
Bio-protocol, 4(22) (2014)
A fast, efficient chromatin immunoprecipitation method for studying protein-DNA binding in Arabidopsis mesophyll protoplasts.
Plant methods, 13(1), 42-42 (2017)
Differentiation of ruminant transmissible spongiform encephalopathy isolate types, including bovine spongiform encephalopathy and CH1641 scrapie.
Jacobs JG, et al.
The Journal of General Virology, 92(1), 222-232 (2011)
R.C. Ogden et al.
Methods in Enzymology, 152, 78-78 (1987)
A new method allowing long-term potentiation recordings in hippocampal organotypic slices.
Paci P, et al.
Brain and Behavior, 7(5), e00692-e00692 (2017)
모든 관련된 서류 보기

프로토콜

Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

Annealing Oligonucleotides Protocol

Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences.

관련 콘텐츠

Trizma Base Specification Comparison

Trizma Base Specification Comparison

Data Sheet - 93362

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