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Merck

A6191

Antipain dihydrochloride

lyophilized powder

동의어(들):

N-(Nα-Carbonyl-Arg-Val-Arg-al)-Phe

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C27H44N10O6 · 2 HCl
CAS 번호:
37682-72-7
Molecular Weight:
677.62
UNSPSC Code:
12352202
PubChem Substance ID:
24891067
NACRES:
NA.77
MDL number:
MFCD00135957

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제품 이름

Antipain dihydrochloride from microbial source, protease inhibitor

SMILES string

Cl[H].Cl[H].[H]C(=O)C(CCCNC(N)=N)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(N)=N)NC(=O)NC(Cc1ccccc1)C(O)=O)C(C)C

InChI key

YAHXZYICKJUJEO-BXLPLHKWSA-N

InChI

1S/C27H44N10O6.2ClH/c1-16(2)21(23(40)34-18(15-38)10-6-12-32-25(28)29)37-22(39)19(11-7-13-33-26(30)31)35-27(43)36-20(24(41)42)14-17-8-4-3-5-9-17;;/h3-5,8-9,15-16,18-21H,6-7,10-14H2,1-2H3,(H,34,40)(H,37,39)(H,41,42)(H4,28,29,32)(H4,30,31,33)(H2,35,36,43);2*1H/t18?,19-,20?,21-;;/m0../s1

biological source

Streptomyces sp.

form

lyophilized powder

potency

1.4 μM Ki

solubility

H2O: 50 mg/mL, 1-butanol: soluble, 1-propanol: soluble, DMSO: soluble, ethanol: soluble, methanol: soluble

mode of action

enzyme | inhibits

storage temp.

−20°C

Quality Level

300

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General description

Chemical structure: peptide

Application

Antipain dihydrochloride from microbial source has been used in the preparation of adipocyte proteins for western blotting and in protease inhibitor cocktail for isolating nuclear extracts for gelshift analysis.
Concentrations for 50% inhibition (μg/ml):
papain, 0.16
trypsin, 0.26
cathepsin A, 1.19
cathepsin B, 0.59
cathepsin D, 125
plasmin, >93
chymotrypsin and pepsin, >250
It also has been reported to inhibit calpain I, (porcine) with Ki = 1.4 μM

Biochem/physiol Actions

Antipain dihydrochloride also inhibits the action of papain and cathespsin B.
Non-selective serine and cysteine protease inhibitor.
Reversible inhibitor of serine/cysteine proteases and some trypsin-like serine proteases. Its action resembles leupeptin; however, its plasmin inhibition is less and its cathepsin A inhibition is more than that observed with leupeptin. Chronic administration of antipain can reduce the frequency of chemically induced transformation in BALB/c-/3T3 cells.

Preparation Note

Solubility testing at 50 mg/ml in water yields a clear to slightly hazy yellow solution. It is reportedly soluble in methanol, water, and DMSO; less soluble in ethanol, butanol, and propanol; insoluble in benzene, hexane, and chloroform.8 A stock solution in water or buffer is stable for about a month at -20 °C.

Dilute solutions should be stored on ice and kept for only a day because of the terminal aldehyde, which is subject to oxidation and racemization.
Stock solutions in water or buffer stable for 1 week at 4 °C, 1 month at −20 °C.

저장 등급

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
078M4146V
069M4068V
039M4067V
12180762
063M4111V

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문서 라이브러리 방문

Dawson, R., ed.
Data for Biochemical Research, 328-328 (1987)
Methods for Protein Analysis: A Practical Guide for Laboratory Protocols, 23-23 (2013)
Beynon, R.J. and Bond, J.S., ed.
Proteolytic Enzymes: A Practical Approach, 242-242 (1989)
The adipose tissue phenotype of hormone-sensitive lipase deficiency in mice
Wang S, et al.
Obesity Research, 9(2), 119-128 (2001)
Wenjie Xia et al.
Foods (Basel, Switzerland), 9(6) (2020-07-02)
In this study, a novel method called selective proteolysis was applied to the glycinin component of soy protein isolate (SPI), and a degraded glycinin hydrolysate (DGH) was obtained. The effects of high-intensity ultrasound (HIU) treatment (20 kHz at 400 W
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