A8312
m-Aminophenylboronic acid–Agarose
aqueous suspension
동의어(들):
(3-aminophenyl)boronic acid-Agarose, 3-Aminophenylboronic acid–Agarose, m-APBA-Agarose, m-Aminophenylboronic acid resin
SMILES string
[X]Nc1cc(ccc1)B(O)O
form
aqueous suspension
extent of labeling
40-80 μmol per mL
matrix
6% beaded agarose
matrix activation
epichlorohydrin
matrix attachment
through amino to carboxyls of EDTA
matrix spacer
9 atoms
capacity
≥8 mg/mL binding capacity (peroxidase Type VI)
storage temp.
2-8°C
Quality Level
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General description
m-Aminophenylboronic acid (m-APBA) immobilized on an agarose gel is useful in immunoglobulins capture, but also leads to non-specific interactions. m-APBA is a boronate affinity matrix that is effectively used for affinity purification of monoclonal antibodies.
Application
m-Aminophenylboronic acid-Agarose has been used:
- as a component of the column for the purification of Escherichia coli TOP10F cells
- with cell lysates of various cells for detection of PARylated (Poly(ADP-ribos)ylation) proteins.
- in the affinity purification of horseradish peroxidase (HRPO)
- in the glycated human serum albumin (gHSA) purification
Physical form
Suspension in water containing 0.002% chlorhexidine diacetate.
저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
T A Myöhänen et al.
The Biochemical journal, 197(3), 683-688 (1981-09-01)
The synthesis of 3-nitro-4-(6-aminohexylamido)phenylboronic acid is described. The properties of two novel forms of immobilized phenylboronate agarose adsorbents [m-aminophenylboronic acid-Matrex Gel and 3-nitro-4-(6-aminohexylamido)phenylboronic acid-Sepharose CL-6B] were investigated. Both gels bind and selectively retard the glycoprotein alpha-glucosidase from yeast. The retardation
Construction and optimization of a CC49-Based scFv-beta-lactamase fusion protein for ADEPT
Roberge M, et al.
Protein engineering, design & selection : PEDS, 19(4), 141-145 (2006)
RUNX poly (ADP-ribosyl) ation and BLM interaction facilitate the Fanconi anemia pathway of DNA repair
Tay L S, et al.
Testing, 24(7), 1747-1755 (2018)
Cai Zhang et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 25(22), 6827-6838 (2019-08-07)
Despite the FDA approval of mTOR inhibitors (mTORi) for the treatment of renal cell carcinoma (RCC), the benefits are relatively modest and the few responders usually develop resistance. We investigated whether the resistance to mTORi is due to upregulation of
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