Fluoroshield^™ with DAPI

Fluoroshield with DAPI is an aqueous mounting medium fortified with 4′,6-diamidino-2-phenylindole (DAPI), a nuclear counterstain used as a blue-fluorescent DNA probe. It is an anti-fading agent employed for preserving the fluorescence of tissue and cell smears by preventing rapid photobleaching and minimizing the blinking-mediated phenomena.(1) For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

Fluoroshield^™ with DAPI has been used:

• as a counterstain in immunocytochemistry to label nuclei of neural progenitor cells
• as a histology mounting medium for cardiac tissues sections for immunofluorescence studies
• as mounting solution for immunostained heart sections for nuclei detection

FluoroShield is a trademark of ImmunoBioScience Corp.