제품 이름
Fluoroshield™ with DAPI, liquid
Quality Level
description
histology mounting medium
form
liquid
composition
Sodium Azide, ≤1% (also contains other ingredients), Tris-HCl
technique(s)
immunofluorescence: suitable
pH range
7.9-8.3
solubility
water: soluble
fluorescence
λex 360 nm; λem 460 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
General description
Fluoroshield with DAPI is an aqueous mounting medium fortified with 4′,6-diamidino-2-phenylindole (DAPI), a nuclear counterstain used as a blue-fluorescent DNA probe. It is an anti-fading agent employed for preserving the fluorescence of tissue and cell smears by preventing rapid photobleaching and minimizing the blinking-mediated phenomena.(1) For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.
Application
Fluoroshield™ with DAPI has been used:
- as a counterstain in immunocytochemistry to label nuclei of neural progenitor cells
- as a histology mounting medium for cardiac tissues sections for immunofluorescence studies
- as mounting solution for immunostained heart sections for nuclei detection
Legal Information
FluoroShield is a trademark of ImmunoBioScience Corp.
저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
문서
Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.
High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.
Extracellular matrix-derived extracellular vesicles promote cardiomyocyte growth and electrical activity in engineered cardiac atria
An M, et al.
Biomaterials, 146(1), 49-59 (2017)
Maria L Spletter et al.
eLife, 7 (2018-05-31)
Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric components group
M Herbette et al.
DNA repair, 57, 139-150 (2017-08-07)
Maintaining the integrity of genetic information across generations is essential for both cell survival and reproduction, and requires the timely repair of DNA damage. Histone-modifying enzymes play a central role in the DNA repair process through the deposition and removal