F9037
Formamide
≥99.5% (GC), BioReagent, Molecular Biology
동의어(들):
Amide C1, Formic amide
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
HCONH2
CAS 번호:
Molecular Weight:
45.04
UNSPSC Code:
12352111
NACRES:
NA.52
PubChem Substance ID:
EC Number:
200-842-0
Beilstein/REAXYS Number:
505995
MDL number:
Assay:
≥99.5% (GC)
Grade:
Molecular Biology
Technique(s):
electrophoresis: suitable, immunohistochemistry: suitable
Bp:
210 °C (lit.)
Vapor pressure:
0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
SMILES string
NC=O
InChI key
ZHNUHDYFZUAESO-UHFFFAOYSA-N
InChI
1S/CH3NO/c2-1-3/h1H,(H2,2,3)
grade
Molecular Biology
vapor density
1.55 (vs air)
vapor pressure
0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
product line
BioReagent
assay
≥99.5% (GC)
form
liquid
autoignition temp.
932 °F
technique(s)
electrophoresis: suitable, immunohistochemistry: suitable
refractive index
n20/D 1.447 (lit.)
pH
4-10 (20 °C, 200 g/L)
bp
210 °C (lit.)
mp
2-3 °C (lit.)
density
1.134 g/mL at 25 °C (lit.)
suitability
suitable for nucleic acid hybridization
storage temp.
2-8°C
Quality Level
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Application
- nucleic acid hybridization
- cryoprotectant and gel-stabilizer
- destabilize nucleic acid duplexes
- elimination of secondary structure of nucleic acids
- preparation of hybridization buffers for RNA and DNA application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Formamide is suitable for use in hybridization buffers made using standard recipes. Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Other applications include:
Other applications include:
- Preparation of brain sections of mice for BrdU immunohistochemistry
- FISH analysis of HeLa cells.
- Preparation of the polyacrylamide gel for analysis of PCR products using denaturing gradient gel electrophoresis(The Merck Index, 12th ed).
Features and Benefits
- Deionized for molecular biology applications
- Packaged under argon gas in amber bottles to protect from oxidation
- Used for DNA renaturation or DNA-RNA hybridization
- Reduces thermal stability of double stranded nucleic acids
General description
Formamide is a clear liquid amide derived from formic acid. Formamide allows for the denaturation and renaturation of nucleic acids at room temperature.
Other Notes
The product has been deionized for molecular biology use and packaged under argon gas in amber glass bottles to protect it from oxidation.
signalword
Danger
hcodes
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
target_organs
Blood
저장 등급
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
305.6 °F
flash_point_c
152 °C
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Laurent Geiser et al.
Journal of chromatography. A, 979(1-2), 389-398 (2002-12-25)
A nonaqueous capillary electrophoresis (NACE) method, coupled with either UV or electrospray mass spectrometry (ESI-MS), is described for the simultaneous analysis of seven beta-blockers. The same electrolyte, namely 25 mM ammonium formate and 1 M formic acid, was used with
R Temmerman et al.
Applied and environmental microbiology, 69(1), 220-226 (2003-01-07)
In order to obtain functional and safe probiotic products for human consumption, fast and reliable quality control of these products is crucial. Currently, analysis of most probiotics is still based on culture-dependent methods involving the use of specific isolation media
T Kaabache et al.
Analytical biochemistry, 232(2), 225-230 (1995-12-10)
The sensitivity of direct solution hybridization of hepatocytes solubilized in guanidium thiocyanate (GuSCN) for detecting alpha 1-acid glycoprotein and albumin mRNAs was studied. The sensitivity of detection was inversely correlated with the DNA concentration. Raising the hybridization temperature from 20
Jianwei Li
Journal of pharmaceutical sciences, 91(8), 1873-1879 (2002-07-13)
This article describes the proper selection of extraction solvents to eliminate interference from a polymer matrix to the quantitation of estradiol degradation products in a transdermal formulation by reversed-phase liquid chromatography. The separation is performed by gradient elution with acetonitrile
N C Pagratis
Nucleic acids research, 24(18), 3645-3646 (1996-09-15)
Streptavidin induced electrophoretic mobility shift was used to prepare single stranded (ss) DNA amplified with the polymerase chain reaction in the presence of a biotinylated and a non-biotinylated primer. A variety of denaturing conditions, including incubation at 95 degrees C
관련 콘텐츠
Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.
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