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Merck

G6795

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal

clone GSN24, purified from hybridoma cell culture

동의어(들):

Anti-GFP

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NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
GSN24, monoclonal
Application:
WB
Citations:
31
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GSN24, monoclonal

form

buffered aqueous solution

mol wt

antigen 27 kDa

concentration

~2 mg/mL

technique(s)

western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C, 2-8°C

target post-translational modification

unmodified

General description

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.

Application

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.

Biochem/physiol Actions

Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.
The antibody reacts specifically with GFP fusion proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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저장 등급

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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관련 콘텐츠

Instructions


Marta Ukleja et al.
Nature communications, 7, 10433-10433 (2016-01-26)
CCR4-NOT is a large protein complex present both in cytoplasm and the nucleus of eukaryotic cells. Although it is involved in a variety of distinct processes related to expression of genetic information such as poly(A) tail shortening, transcription regulation, nuclear
Piet Kramer et al.
Frontiers in genetics, 7, 165-165 (2016-09-30)
The mitochondrial permeability transition pore plays a key role in programmed cell death and the induction of autophagy. Opening of the pore is regulated by the mitochondrial peptidyl prolyl-cis, trans-isomerase cyclophilin D (CYPD). Previously it was shown in the aging
Matthias Wiemer et al.
F1000Research, 3, 230-230 (2014-12-19)
The degradation of damaged proteins is an important vital function especially during aging and stress. The ubiquitin proteasome system is one of the major cellular machineries for protein degradation. Health and longevity are associated with high proteasome activity. To demonstrate