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H7523

HEPES

BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

동의어(들):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C8H18N2O4S
CAS 번호:
7365-45-9
Molecular Weight:
238.30
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24895796
EC Number:
230-907-9
Beilstein/REAXYS Number:
883043
MDL number:
MFCD00006158
기술 서비스
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도움 문의

product line

BioXtra

Quality Level

400

assay

≥99.5% (titration)

form

powder

impurities

Insoluble matter, passes filter test

ign. residue (900°C)

≤0.1% (as SO4)

loss

≤1.0% loss on drying, 110°C

pH

5.0-6.5 (1 M in H2O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.05%

cation traces

Al: ≤0.0005%, As: ≤0.0001%, Ba: ≤0.0005%, Bi: ≤0.0005%, Ca: ≤0.001%, Cd: ≤0.0005%, Co: ≤0.0005%, Cr: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Li: ≤0.0005%, Mg: ≤0.0005%, Mn: ≤0.0005%, Mo: ≤0.0005%, Na: ≤0.005%, Ni: ≤0.0005%, Pb: ≤0.0005%, Sr: ≤0.0005%, Zn: ≤0.0005%

absorption

≤0.035 at 280 in H2O at 1 M, ≤0.045 at 260 in H2O at 1 M

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • As a component of homogenisation buffer used in protein sample preparation
  • To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
  • As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
  • As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

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flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

저장 등급

11 - Combustible Solids

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시험 성적서(COA)

Lot/Batch Number

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HEPES Specification Comparison

HEPES Specification Comparison