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Merck

M0644

Minimum Essential Medium Eagle

Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture

동의어(들):

MEM

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NACRES:
NA.75
UNSPSC Code:
12352207
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Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.

Application

Minimum essential medium Eagle has been used to:

  • maintain natural killer (NK) cell lines
  • culture Leishmania donovani strains
  • culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles

Preparation Note

Formulated to contain 10.1 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.


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Hazard Classifications

Skin Sens. 1

저장 등급

11 - Combustible Solids

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Not applicable

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Not applicable



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