P4557
Phenol solution
Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, Molecular Biology
동의어(들):
Tris-equilabrated Phenol
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C6H6O
CAS 번호:
Molecular Weight:
94.11
UNSPSC Code:
12352104
NACRES:
NA.52
PubChem Substance ID:
EC Number:
203-632-7
Beilstein/REAXYS Number:
6428463
MDL number:
Assay:
≥99.0% (GC)
Grade:
Molecular Biology
InChI key
ISWSIDIOOBJBQZ-UHFFFAOYSA-N
InChI
1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
SMILES string
Oc1ccccc1
grade
Molecular Biology
product line
BioReagent
assay
≥99.0% (GC)
form
liquid
dilution
(for analytical testing)
pH
7.7-8.1 (25 °C)
storage temp.
2-8°C
Quality Level
Gene Information
human ... GABRA1(2554)
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Application
Phenol solution has been used:
- as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
- for extraction of DNA from Arabidopsis thaliana Rosette leaves
- in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
- as a component of extraction buffer to extract RNA from fruit pulp
- for the extraction of protein from roots
- for the isolation of fecal DNA
- for the extraction of caged RNA
Biochem/physiol Actions
The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol.
General description
A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.
Other Notes
- 1 bottle Tris-equilabrated Phenol (product number P4557)
- Equilibration Buffer (product B5658)
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
저장 등급
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
Michael B Powner et al.
Nature protocols, 7(6), 1086-1096 (2012-05-12)
The mouse retinal vasculature provides a powerful model system for studying development and pathologies of the vasculature. Because it forms as a two-dimensional flat plexus, it is easily imaged in its entirety in whole-mount retinal preparations. In order to study
Debby Bogaert et al.
PloS one, 6(2), e17035-e17035 (2011-03-10)
The nasopharynx is the ecological niche for many commensal bacteria and for potential respiratory or invasive pathogens like Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis. Disturbance of a balanced nasopharyngeal (NP) microbiome might be involved in the onset of symptomatic
Fecal Microbiome and Food Allergy in Pediatric Atopic Dermatitis: A Cross-Sectional Pilot Study
Fieten KB, et al.
International Archives of Allergy and Immunology, 175(1-2), 77-84 (2018)
Tim J Bull et al.
Journal of clinical microbiology, 41(7), 2915-2923 (2003-07-05)
Mycobacterium avium subsp. paratuberculosis is a robust and phenotypically versatile pathogen which causes chronic inflammation of the intestine in many species, including primates. M. avium subsp. paratuberculosis infection is widespread in domestic livestock and is present in retail pasteurized cows'
Thitiporn Anunthawan et al.
Biochimica et biophysica acta, 1848(6), 1352-1358 (2015-03-15)
We investigated the mechanisms of two tryptophan-rich antibacterial peptides (KT2 and RT2) obtained in a previous optimization screen for increased killing of both Gram-negative and Gram-positive bacteria pathogens. At their minimal inhibitory concentrations (MICs), these peptides completely killed cells of
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