grade
Molecular Biology
description
Saline-Sodium Citrate buffer, made with ultrapure water
sterility
0.2 μm filtered
form
solution
Quality Level
color
colorless
pH
6.9 —7.1
application(s)
diagnostic assay manufacturing
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
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Application
SSC Buffer 20× Concentrate has been used
- as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
- as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
- as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon
Features and Benefits
- Convenient ready-to-use liquid
- Multi-purpose use in hybridizations
General description
SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.
Preparation Note
SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Chronic hypoxia facilitates Alzheimer's disease through demethylation of gamma-secretase by downregulating DNA methyltransferase 3b
Liu H, et al.
Alzheimers Dement., 12(2), 130-143 (2016)
L1 retrotransposon expression in circulating tumor cells
Papasotiriou I, et al.
Testing, 12(2), e0171466-e0171466 (2017)
Michaela Asp et al.
Scientific reports, 7(1), 12941-12941 (2017-10-13)
Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In
Chang-Gong Liu et al.
Methods (San Diego, Calif.), 44(1), 22-30 (2007-12-26)
After 12 years from its first application, microarray technology has become the reference technique to monitor gene expression of thousands of genes in the same experiment. In the past few years an increasing amount of evidence showed the importance of
M Böttger et al.
Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
문서
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
사용 가능한 형광 동소 교잡법(FISH) 절차, 시약, 장비.
프로토콜
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.