S8761
Sodium Bicarbonate
Solution (7.5%), sterile-filtered, suitable for cell culture
동의어(들):
aqueous sodium bicarbonate
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제품 이름
Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture
sterility
sterile-filtered
Quality Level
product line
BioReagent
form
solution (7.5%)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
7.8-8.2
storage temp.
2-8°C
SMILES string
[Na+].OC([O-])=O
InChI
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
InChI key
UIIMBOGNXHQVGW-UHFFFAOYSA-M
General description
Sodium bicarbonate (NaHCo3) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.
Application
Sodium bicarbonate solution has been used:
- as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice
- as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants
- as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma
- to prepare collagen mix droplets for 3D collagen sprouting assay
- to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture
Preparation Note
Prepared in cell culture grade water.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
프로토콜
3-D Culture Matrix™ Collagen I facilitates cellular growth and differentiation in vitro, offering versatile applications in biomedical research.