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Merck

T8154

Trypan Blue solution

0.4%, liquid, sterile-filtered, suitable for cell culture

동의어(들):

Blue cell viability dye

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C34H24N6Na4O14S4
CAS 번호:
72-57-1
Molecular Weight:
960.81
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
57654704
MDL number:
MFCD00003969
Beilstein/REAXYS Number:
4360496

주요 문서

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InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

sterility

sterile-filtered

form

liquid

storage condition

dry at room temperature

concentration

0.4%

technique(s)

cell culture | mammalian: suitable, tissue processing: suitable

application(s)

cell analysis

shipped in

ambient

Quality Level

200

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관련 카테고리

Application

Trypan Blue solution has been used:
  • in cell viability assay to count viable and dead cells
  • in rescue assay to count viable cells
  • in trypan blue exclusion counting method to determine proliferation curve
  • as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
  • in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
  • to count GEC cells using a hemocytometer

General description

For the same product in a convenient lyophilized (powder) format, please see T6146

Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.
Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.

Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

저장 등급

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
RNBM0108
RNBL9613
RNBL9248
RNBL8011
RNBL7714

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