581325-U
Ascentis® C18 (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
동의어(들):
Ascentis RP18 HPLC Column
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제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
C18 (RP18), C18 (octadecyl) phase
Pore size:
100 Å
Matrix:
fully porous particle, silica gel high purity, spherical
제품 이름
Ascentis® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
Quality Segment
agency
suitable for USP L1 (Similar to Phenomenex Luna C18)
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
extent of labeling
25% Carbon loading
parameter
≤70 °C temp. range, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable, LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
surface area
450 m2/g
surface coverage
3.7 μmol/m2
impurities
<5 ppm metals
matrix
fully porous particle, silica gel high purity, spherical
matrix active group
C18 (RP18), C18 (octadecyl) phase
particle size
5 μm
pore size
100 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
General description
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.
Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.
Application
- Identification of recombinant human insulin and biosynthetic insulin analogues by multiplexed targeted unlabeled mass spectrometry of proteotypic tryptic peptides.: This study utilized the Ascentis® C18 HPLC column for the separation of proteotypic tryptic peptides in the identification of insulin analogues, highlighting its application in targeted mass spectrometry for proteomic analysis (Qasem RJ et al., 2019).
- Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben and propylparaben in a topical cream preparation.: This research developed and validated an HPLC method using the Ascentis® C18 HPLC column for the simultaneous assay of multiple compounds, demonstrating its robustness in stability testing of pharmaceutical formulations (Byrne J et al., 2014).
- HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.: The study employed the Ascentis® C18 HPLC column for metabolite profiling of propolis extracts using HPLC-DAD and HPLC-ESI-MS/MS, showcasing its effectiveness in complex natural product analysis (Pellati F et al., 2011).
- Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.: This article compared different HPLC column technologies, including the Ascentis® C18, highlighting its performance in fast separations critical for pharmaceutical process development (Abrahim A et al., 2010).
Features and Benefits
- Excellent retention
- Symmetric peak shape
- High reproducibility
- Complete LC-MS compatibility
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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저장 등급
11 - Combustible Solids
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