268298
DAPI, Dihydrochloride
Cell-permeable DNA-binding dye.
Synonym(s):
DAPI, Dihydrochloride, 4ʹ,6-Diamidino-2-phenylindole, 2HCl
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About This Item
Empirical Formula (Hill Notation):
C16H15N5 · 2HCl
CAS Number:
Molecular Weight:
350.25
UNSPSC Code:
12352200
NACRES:
NA.54
MDL number:
Quality Segment
description
RTECS - NL5995050
assay
≥90% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic), protect from light
color
yellow
solubility
water: 5 mg/mL, ethanol: soluble, methanol: soluble
fluorescence
λex ~359 nm, λem ~461 nm
shipped in
ambient
storage temp.
−20°C
SMILES string
[Cl-].[Cl-].[nH]1c2c(cc1c3ccc(cc3)C(=N)N)ccc(c2)C(=N)N.[H+].[H+]
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
General description
Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H2O (Cat. No. 508741).
Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Binds preferentially to DNA rich in adenine and thymine
Binds preferentially to DNA rich in adenine and thymine
Product does not compete with ATP.
Reversible: yes
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
signalword
Warning
hcodes
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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