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Merck

492016

NP-40 Alternative

Synonym(s):

NP-40 Alternative - CAS 9016-45-9 - Calbiochem, Nonylphenyl Polyethylene Glycol

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About This Item

CAS Number:
9016-45-9
UNSPSC Code:
12161902
NACRES:
NA.25
MDL number:
MFCD00132851

Key Documents

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SMILES string

O(CCOc1ccc(cc1)CCCCCCCCC)CCO

InChI

1S/C19H32O3/c1-2-3-4-5-6-7-8-9-18-10-12-19(13-11-18)22-17-16-21-15-14-20/h10-13,20H,2-9,14-17H2,1H3

InChI key

BLXVTZPGEOGTGG-UHFFFAOYSA-N

description

RTECS - MD0905000

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated

color

clear colorless

CMC

50 - 300 μM

solubility

water: 25 mg/mL, aqueous buffer: soluble

density

1.06 g/mL

application(s)

(Isolating membrane proteins)

shipped in

ambient

storage temp.

15-25°C

Quality Level

200

Related Categories

General description

A non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis. Similar in usage to NP-40 and TRITON™ X-100 Detergent(Cat. No. 648462).

Application

NP-40 Alternative has been used:
  • as a component of the lysis buffer for solubilizing proteins from transfected HEK293 cells.
  • as a component of the wash buffer for efficient solubilization of biotinylated plasma proteins in plasma protein profiling and chromatin immunoprecipitation (ChIP).
  • for the purification of doublet microtubules from cilia for single particle cryo-electron microscopy (cryo-EM).

Other Notes

Ripoche, P., et al. 1982. Biochim. Biophys. Acta693, 497.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

Toxicity: Irritant (B)

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Danger

Storage Class

11 - Combustible Solids

flash_point_f

503.6 °F - Pensky-Martens closed cup - (own results)

flash_point_c

262 °C - Pensky-Martens closed cup - (own results)

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2

Certificates of Analysis (COA)

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Susan H Eshleman et al.
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This study evaluates HIV antibody responses and their evolution during the course of HIV infection. A phage display system is used to characterize antibody binding to >3,300 HIV peptides in 57 adults with early- to late-stage infection. We find that
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Chemical & pharmaceutical bulletin, 67(9), 966-976 (2019-07-02)
Honokiol, a biphenolic neolignan isolated from Magnolia officinalis, was reported to have a promising anti-inflammatory activity for the treatment of various diseases. There are many efforts on the synthesis and structure-activity relationship of honokiol derivatives. However, regioselective O-alkylation of honokiol
Peiwei Liu et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(5), E934-E943 (2018-01-18)
Bardet-Biedl syndrome (BBS) is a ciliopathy resulting from defects in the BBSome, a conserved protein complex. BBSome mutations affect ciliary membrane composition, impairing cilia-based signaling. The mechanism by which the BBSome regulates ciliary membrane content remains unknown. Chlamydomonas bbs mutants
Arnab Mukhopadhyay et al.
Nature protocols, 3(4), 698-709 (2008-04-05)
In order to determine how signaling pathways differentially regulate gene expression, it is necessary to identify the interactions between transcription factors (TFs) and their cognate cis-regulatory DNA elements. Here, we have outlined a chromatin immunoprecipitation (ChIP) protocol for use in
Gustav Roder et al.
European journal of immunology, 39(10), 2682-2694 (2009-09-04)
Tapasin (Tpn) is an ER chaperone that is uniquely dedicated to MHC-I biosynthesis. It binds MHC-I molecules, integrates them into peptide-loading complexes, and exerts quality control of the bound peptides; only when an "optimal peptide" is bound will the MHC-I
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