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507861

PANSORBIN® Cells, Standardized

Synonym(s):

Cell standard

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About This Item

NACRES:
NA.42
UNSPSC Code:
41116133
Manufacturer/tradename:
Calbiochem®
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form

liquid

Quality Segment

100

contains

0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

capacity

≥2 mg/mL binding capacity

shipped in

ambient

storage temp.

2-8°C

General description

Heat-killed, formalin-fixed Staphylococcus aureus cells (Cowan I strain) that bear a high cell-surface density of protein A and have been pickled by the method of Kessler. Useful as a solid-phase IgG-binding reagent due to the high-affinity interaction of protein A with the Fc domain of IgG. PANSORBIN cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3).
PANSORBIN Cells are heat-killed, formalin fixed Staphylococcus aureus cells that have a coat of protein A and have been pickled by the method of Kessler. Useful for mitogenic stimulation of B lymphocytes and for immunoprecipitation.

Application

Most common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes.

Physical form

Supplied as a ≥10% (w/v) Staphylococcus aureus cell suspension in PBS, 0.1% sodium azide, pH 7.2.

Other Notes

Kierszenbaum, F., et al. 1991. Immunology74, 317.
Meikle, P.J., et al. 1991. J. Biol. Chem.266, 22569.
Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
Murakami, H., et al. 1988. Biochem. J.256, 917.
Kessler, S.W. 1975. J. Immunol. 115, 1617.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PANSORBIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

12 - Non Combustible Liquids

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