MAB1951Z
Anti-Integrin beta 1 (CD29) Antibody
CHEMICON®, mouse monoclonal, P4G11
Synonym(s):
CD29, MAB1951
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
P4G11, monoclonal
Species reactivity:
monkey, human, rabbit
Application:
ELISA, FACS, ICC, IHC, IP
Citations:
25
Product Name
Anti-Integrin β1 Antibody, clone P4G11, azide free, clone P4G11, Chemicon®, from mouse
biological source
mouse
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
P4G11, monoclonal
species reactivity
monkey, human, rabbit
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ITGB1(3688)
Immunogen
Human IL-1beta activated endothelial cells
Application
Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}
Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.
Traditional formalin fixation is not recommended.
Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)
Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)
FACS: 2-5μg per 10e6 cells, in the presence of Ca++.
Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.
ELISA
Optimal working dilutions must be determined by end user.
Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.
Traditional formalin fixation is not recommended.
Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)
Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)
FACS: 2-5μg per 10e6 cells, in the presence of Ca++.
Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
This Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1.
Biochem/physiol Actions
Human integrin beta1. The epitope recognized by MAB1951Z is Ca2+ dependent.
Physical form
Format: Purified
Sterile-filtered protein A purified immunoglobulin in 0.01M PBS, pH 7.1, containing no preservatives.
Preparation Note
Maintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Still not finding the right product?
Explore all of our products under Anti-Integrin β1 Antibody, clone P4G11, azide free
— or —
Try our Product Selector Tool to narrow your options
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.