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70134

Brilliant green agar, modified

suitable for microbiology, NutriSelect® Basic

Synonym(s):

Brilliant Green Phenol Red Lactose Sucrose Agar, modified

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171606
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sterility

non-sterile

Quality Level

100

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12.0 g/L , bacteriological peptone, 10.0 g/L , brilliant green, 0.0047 g/L , disodium hydrogen phosphate, 1.0 g/L , lactose, 10.0 g/L , meat extract, 5.0 g/L , phenol red, 0.09 g/L , sodium dihydrogen phosphate, 0.6 g/L , sucrose, 10.0 g/L , yeast extract, 3.0 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

6.9±0.2 (25 °C)

application(s)

environmental
food and beverages
water monitoring, microbiology

suitability

selective and differential for Salmonella spp.

Application

A highly selective medium for the isolation and identification of Salmonella except S. typhosa or Shigella species.

Preparation Note

Suspend 52 g in 1 litre of distilled water. Heat gently with occasional agitation and bring just to the boil to dissolve the medium completely. Do not autoclave! Cool to 50°C, mix well and pour plates.

Other Notes

Salmonella isolation in 9 European laboratories using a standardized technique; Variation in plating efficiency of Salmonellae in eight lots of Brilliant Green Agar
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

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R B Read et al.
Applied microbiology, 16(5), 746-748 (1968-05-01)
The plating efficiency of Salmonella anatum, S. cubana, S. dublin, S. tennessee, and S. typhimurium was determined for eight lots of Brilliant Green Agar made by two manufacturers. Washed cells were used as the inoculum and cultures were incubated at
K D Teague et al.
Poultry science, 96(7), 2074-2082 (2017-02-06)
Three experiments were conducted to evaluate the effect of in ovo administration of FloraMax®-B11 (FM) on Marek's disease (MD) herpesvirus of turkeys (HVT) vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and Salmonella enteritidis (SE) infection in chickens. Experiment 1
Omar F Prado-Rebolledo et al.
Avian pathology : journal of the W.V.P.A, 46(1), 90-94 (2016-08-23)
Two experiments were conducted to evaluate the effect of a lactic acid bacteria-based probiotic (FloraMax-B11
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