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Merck

M8302

MacConkey Agar

with Crystal violet, Sodium chloride and 0.15% Bile salts, NutriSelect® Plus, according to USP, powder, selective for enterobacteriaceae, pack of 500 g, suitable for microbiology

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Plus
Agency:
according to USP
Ph:
7.2±0.2 (25 °C)
Form:
powder
Suitability:
selective and differential for Enterobacter spp., selective and differential for Enterococcus spp., selective and differential for Escherichia coli, selective and differential for Klebsiella spp., selective and differential for Proteus spp., selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for Streptococcus spp., selective and differential for coliforms, selective for enterobacteriaceae
Application(s):
environmental
food and beverages, microbiology

Key Documents

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Quality Level

200

agency

according to USP

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

with Crystal violet, Sodium chloride and 0.15% Bile salts

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

pH

7.2±0.2 (25 °C)

application(s)

environmental
food and beverages, microbiology

suitability

selective and differential for Enterobacter spp., selective and differential for Enterococcus spp., selective and differential for Escherichia coli, selective and differential for Klebsiella spp., selective and differential for Proteus spp., selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for Streptococcus spp., selective and differential for coliforms, selective for enterobacteriaceae

Related Categories

Application

A differential media recommended for the selection and recovery of Enterobacteriaceae.

Other Notes

Components (g/L)
Peptic digest of animal tissue 20.0
Lactose 10.00
Bile salts 1.50
Sodium chloride 5.00
Crystal violet 0.001
Neutral red 0.05
Agar 15.00
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 51.55 g of MacConkey Agar with Crystal Violet, Sodium Chloride and 0.15% Bile Salts in 1000 ml of distilled water. Heat to boiling, with gentle swirling to dissolve the agar completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C and pour into sterile petri plates.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG3230
BCCG3247
BCCF6219
BCCC6759
BCCB7690

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United States Pharmacopeia/National Formulary (1985)
Caroline S Achard et al.
Microorganisms, 8(10) (2020-10-18)
Antibiotic resistance of microbes thriving in the animal gut is a growing concern for public health as it may serve as a hidden reservoir for antibiotic resistance genes (ARGs). We compared 16 control piglets to 24 piglets fed for 3
Ilenys M Pérez-Díaz et al.
Food microbiology, 77, 10-20 (2018-10-10)
Limited documentation of the cucumber fermentation microbiome has impeded the understanding of the role of microbes on the quality of finished products. We characterized the microbiome of fresh and fermented cucumber samples using culture dependent and independent techniques, with an

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