A0295
Anti-Human IgA (α-chain specific)−Peroxidase antibody produced in goat
affinity isolated antibody
Synonym(s):
Goat Anti-Human IgA (α-chain specific)−HRP
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About This Item
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p), WB
Species reactivity:
human
Citations:
45
Technique(s):
direct ELISA: 1:50,000, dot blot: 1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot: suitable
biological source
goat
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human
concentration
4-11 mg/mL
technique(s)
direct ELISA: 1:50,000, dot blot: 1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot: suitable
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
Anti-Human IgA (α-chain specific) Peroxidase antibody produced in goat has been used in:
- Enzyme linked immunosorbent assays (ELISA) at dilution of 1:3,000 and 1: 1,600
- Tissue anti-transglutaminase antibody (tTG) assay at a dilution of 1:1,000
- Dot blot: a minimum working dilution of 1:80,000 was determined in a direct chemiluminescence assay using 10ng human IgA/dot. Luminol plus enhancer was used as substrate.
- Immunohistology using formalin-fixed, paraffin-embedded sections at a dilution of 1:100
- Transcytosis assay of dimeric IgA (D-IgA)
- Western Blotting
Biochem/physiol Actions
Anti-Human IgA (a-chain specific)-Peroxidase antibody produced in goat conjugate is specific for human IgA when tested against human IgA, IgG, IgM, Bence Jones Kappa and Lambda myeloma proteins. The antibody conjugate does not react with mouse or rat IgG.
IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa and prevent the commensal microorganisms from invading the intestines.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-Human IgA (a-chain specific)-peroxidase antibody is isolated from goat anti-human IgA antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the alpha chain of human IgA.Goat antihuman IgA is conjugated to peroxidase and purified to remove unconjugated material. Specificity of the peroxidase conjugated anti-human IgA is determined by immunoelectrophoresis (IEP) and cross-reactivity of the conjugate is determined by enzyme linked immunoassays (ELISA).
Immunogen
Purified human IgA.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT as preservative
Preparation Note
For continuous use, store at 2-8°C for a maximum of one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
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