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Merck

A9226

Alkaline buffer solution

1.5 M, pH 10.3 (25 °C)

Synonym(s):

Basic pH buffer

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About This Item

NACRES:
NA.25
UNSPSC Code:
12161700
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form

liquid

Quality Level

concentration

1.5 M

pH

10.3 (25 °C)

storage temp.

2-8°C

General description

Alkaline buffer solution, 1.5 M, pH 10.3 at 25 °C is used in alkaline phosphatase reactions. It contains 2-amino-2-methylpropanol (AMP) and is used in conjunction with p-nitrophenyl phosphate (pNPP) enzyme substrate.

Application

Alkaline buffer solution has been used to measure the alkaline phosphatase (ALP) activity in the:
  • rat bone marrow stromal cells (BMSC)
  • osteoblast-like cells (SaoS-2, ATCC HTB 85)
  • mouse MC3T3-E1

Other Notes

Contains 2-amino-2-methylpropanol


pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Young-Hee Kim et al.
BMC pharmacology & toxicology, 14, 60-60 (2013-12-03)
Indoxyl sulfate (IS), an organic anion uremic toxin, promotes the progression of renal dysfunction. Some studies have suggested that IS inhibits osteoclast differentiation and suppresses parathyroid hormone (PTH)-stimulated intracellular cAMP production, decreases PTH receptor expression, and induces oxidative stress in
Salima Nedjari et al.
Scientific reports, 7(1), 15947-15947 (2017-11-23)
Stem cells therapy offers a viable alternative for treatment of bone disorders to the conventional bone grafting. However clinical therapies are still hindered by the insufficient knowledge on the conditions that maximize stem cells differentiation. Hereby, we introduce a novel
Helle Lysdahl et al.
BioResearch open access, 3(6), 278-285 (2014-12-04)
Clinical trials using bone morphogenetic protein-2 (BMP2) for bone reconstruction have shown promising results. However, the relatively high concentration needed to be effective raises concerns for efficacy and safety. The aim of this study was to investigate the osteogenic effect