C8541
Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse
clone CY-90, ascites fluid
Synonym(s):
Monoclonal Anti-Cytokeratin Peptide 18
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About This Item
Conjugate:
unconjugated
Clone:
CY-90, monoclonal
Application:
IF, WB
Citations:
30
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
CY-90, monoclonal
contains
15 mM sodium azide
species reactivity
wide range
technique(s)
indirect immunofluorescence: 1:800 using formalin-fixed, paraffin-embedded sections of human tissue, western blot: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells. Epithelial cells and their derivatives characteristically contain intermediate filaments (IFs) composed of about 20 related polypeptides with molecular weights between 40,000-69,000. Each epithelial tissue has a specific and stable pattern of expression of some of these cytokeratin subunits. Epithelium derived tumors maintain the expression of the cytokeratins found in the normal tissue of origin. Therefore, carcinomas can be identified and classified by immunocytochemical staining with antibodies that react specifically with cytokeratins.
Monoclonal Anti-Cytokeratin Peptide 18 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cytokeratin 18 (CK18)/keratin 18 is a structural protein and is present in single-layer epithelia. It is located on human chromosome 12q13.
Immunogen
human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.
Application
Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse has been used in:
- immunofluorescence
- fluorescence microscopy
- confocal fluorescence microscopy
- immunolabelling
- histoblots
Biochem/physiol Actions
Keratins help to maintain structural integrity. They also control Fas?mediated apoptosis and regulate cell size and protein synthesis. Cytokeratin 18 (CK18) plays a major role in the development of lung cancer. It acts as a therapeutic target for non-small cell lung cancer (NSCLC). CK-18 is considered as a promising plasma biomarker to identify patients with HELLP (hemolysis, elevated liver enzymes, low platelets) syndrome.
The antibody reacts specifically with a wide variety of simple epithelia (e.g. intestine, respiratory and urinary systems, liver, and glandular epithelia). It does not react with stratified squamous epithelia (e.g. esophagus or epidermis) or with non-epithelial cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Le Ann Blomberg et al.
Molecular reproduction and development, 73(12), 1491-1502 (2006-08-12)
Gastrulation and trophectoderm elongation of the porcine conceptus coincide with peak conceptus estrogen secretion from gestational day 11 to day 12. The current study aim was to identify genes required for elongation by defining the transcriptome profile of this dynamic
Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle, and brain
Jiang Y, et al.
Experimental Hematology, 30(8), 896-904 (2002)
Role of extracellular signal-regulated kinase pathway in RRR-alpha-tocopheryl succinate-induced differentiation of human MDA-MB-435 breast cancer cells
You H, et al.
Molecular Carcinogenesis, 33(4), 228-236 (2002)
Increased plasma levels of CK-18 as potential cell death biomarker in patients with HELLP syndrome
John K, et al.
Cell Death & Disease, 4(10), e886-e886 (2013)
Alexia Nunez-Parra et al.
Chemical senses, 36(9), 771-780 (2011-06-17)
Cell-to-cell metabolic interactions are crucial for the functioning of the nervous system and depend on the differential expression of glucose transporters (GLUTs) and monocarboxylate transporters (MCTs). The olfactory receptor neurons (ORNs) and supporting cells (SCs) of the olfactory epithelium exhibit
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