D9170
Dimethyl sulfoxide
PCR Reagent
Synonym(s):
DMSO
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About This Item
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
UNSPSC Code:
12191502
NACRES:
NA.25
PubChem Substance ID:
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
Assay:
≥99.5%
Technique(s):
PCR: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)
Quality Segment
vapor density
2.7 (vs air)
vapor pressure
0.42 mmHg ( 20 °C)
assay
≥99.5%
form
liquid
autoignition temp.
573 °F
shelf life
Recommended retest period - 2 years
expl. lim.
42 %, 63 °F
packaging
vial of 1 mL
technique(s)
PCR: suitable
impurities
≤0.1% water
water
color
colorless
refractive index
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubility
H2O: miscible (completely)
density
1.10 g/mL (lit.)
suitability
suitable for PCR
application(s)
agriculture
SMILES string
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
General description
Dimethyl sulfoxide is a free radical scavenger and a cryopreservant. It elicits protective effect in double stranded breaks(DSBs). Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination. As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.
Application
Dimethyl sulfoxide has been used as a component of polymerase chain reaction (PCR) mixture and as PCR facilitator.
Other Notes
For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table
Dimethyl Sulfoxide Miscibility/Immiscibility Table
Disclaimer
Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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