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About This Item
NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
M2, monoclonal
Application:
WB
Citations:
5438
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin (Purified IgG1 subclass)
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
purified by
using Protein A
species reactivity
all
concentration
3.8-4.2 mg/mL
technique(s)
western blot: 10 μg/mL (Protein A)
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
General description
Anti-Flag M2 antibody is used for the detection of Flag fusion proteins. This monoclonal antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site. M2, unlike M1 antibody is not Calcium dependent.
F3165 is affinity purified using Protein A resin, so it contains not only the anti-FLAG M2 antibody but also small amounts of native mouse IgG, increasing its sensitivity in most applications.
Method of purification - Protein A
F3165 is affinity purified using Protein A resin, so it contains not only the anti-FLAG M2 antibody but also small amounts of native mouse IgG, increasing its sensitivity in most applications.
Method of purification - Protein A
Immunogen
FLAG; peptide sequence DYKDDDDK
Application
Monoclonal ANTI-FLAG® M2 antibody has been used in:
Browse additional application references in our FLAG® Literature portal.
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- immunofluorescence
- ELISA
- EIA
- chromatin immunoprecipitation
- electron microscopy
- flow cytometry
- supershift assays
Browse additional application references in our FLAG® Literature portal.
Preparation Note
Dilute the antibody solution from 0.5-10 ug/mL in Tris Buffered Saline, pH 8.0, with 3% nonfat milk
Store the undiluted antibody at –20 °C in working aliquots. Repeated freezing and thawing is not recommended.
Note: Overtime, small amounts of purified antibodies can precipitate from solution due to intermolecular hydrophobic interactions. If a precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in Western blot or immunoprecipitation applications.
Note: Overtime, small amounts of purified antibodies can precipitate from solution due to intermolecular hydrophobic interactions. If a precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in Western blot or immunoprecipitation applications.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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