F4049
ANTI-FLAG® M2-FITC antibody, Mouse monoclonal
clone M2, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Monoclonal ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
M2, monoclonal
Application:
IF (d)
Species reactivity:
all
Citations:
78
Technique(s):
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
biological source
mouse
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution
species reactivity
all
concentration
~1 mg/mL
technique(s)
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Application
Antibody is suitable for immunofluorescence, immunocytochemistry, immunohistochemistry, immunoblotting, flow cytometry and electron microscopy. Ideal for use when detection of Flag fusion proteins is required in a murine host, where secondary antibodies could cause cross reactivity.
Learn more product details in ourFLAG® application portal.
Learn more product details in ourFLAG® application portal.
General description
Anti Flag M2-FITC antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is covalently conjugated to flourescein isothiocyanate (FITC). The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.Binding is not Calcium dependent. F4049 is an Affinity purified antibody, increasing sensitivity in most applications.
Physical form
Solution in 10 mM phosphate buffered saline, plus 1% BSA and 0.1% sodium azide, pH 7.4
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Pete Stavropoulos et al.
Journal of immunological methods, 362(1-2), 190-194 (2010-08-31)
The African trypanosome (Trypanosoma brucei) is transmitted by the bite of the tsetse vector to the mammalian bloodstream where it exists as a completely extracellular parasite. As a result of this exposure, the parasite elicits a robust immune response that
Xuechen Lv et al.
Protein & cell, 7(5), 325-337 (2016-04-18)
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Jing Yi et al.
PloS one, 8(10), e78144-e78144 (2013-11-10)
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Emmanuelle Despras et al.
Nature communications, 7, 13326-13326 (2016-11-05)
Translesion polymerase eta (polη) was characterized for its ability to replicate ultraviolet-induced DNA lesions that stall replicative polymerases, a process promoted by Rad18-dependent PCNA mono-ubiquitination. Recent findings have shown that polη also acts at intrinsically difficult to replicate sequences. However
Jun-ichi Asaka et al.
Molecular pharmacology, 71(6), 1487-1493 (2007-03-01)
Multidrug and toxin extrusion 1 (MATE1) has been isolated as an H(+)/organic cation antiporter located at the renal brush-border membranes. Previous studies using rat renal brush-border membrane vesicles indicated that cysteine and histidine residues played critical roles in H(+)/organic cation
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