G5166
PNGase F Glycosidase
Removes N-linked glycans, suitable for mass spectrometry, from Elizabethkingia miricola
Synonym(s):
N-Glycosidase F, Peptide N-glycosidase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Specific activity:
≥20000 units/mg protein
Biological source:
bacterial (Elizabethkingia miricola)
Product Name
PNGase F from Elizabethkingia miricola, buffered aqueous solution
biological source
bacterial (Elizabethkingia miricola)
Quality Level
conjugate
(N-linked)
form
buffered aqueous solution
specific activity
≥20000 units/mg protein
mol wt
36 kDa
shipped in
wet ice
storage temp.
2-8°C
General description
PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.
Application
PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.
Used to deglycosylate protein.
Biochem/physiol Actions
Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
Physical form
Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA
Other Notes
One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.
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Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Glycobiology and Glycoproteomics Brochure