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Merck

H4641

Hanks′ Balanced Salt Solution 10x

Without calcium chloride, magnesium sulfate and sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Hanks’ Balanced Salt solution, HBSS

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207
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Quality Level

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes

shipped in

ambient

storage temp.

room temp

General description

Balanced salt solution (BSS) is crucial in cell culture as it serves as an irrigating, transporting, and diluting agent to maintain the intra- and extracellular osmotic balance and also provides energy for cell metabolism. It provides water and bulk inorganic ions to the cells to maintain the normal cell metabolism. BSS plays a role in the buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Application

Hanks′ Balanced Salt Solution 10x has been used:

  • as a diluting agent during the Chinese hamster ovary cell line detachment from growth medium
  • to wash the biopsy specimens of lamina propria lymphocytes (LPLs)
  • as a wash solution to wash the fish cell line during removal from growth medium

Preparation Note

Supplement with 0.35 g/L sodium bicarbonate at 1X.


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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