H8541
Transforming Growth Factor-β1 human
≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonym(s):
TGF-β1
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About This Item
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
dimer 25 kDa (non-glycosylated)
Impurities:
≤1 EU/μg
Product Name
Transforming Growth Factor-β1 human, TGF-β1, Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture
biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤0.5 ng/mL EC50
quality
endotoxin tested
mol wt
dimer 25 kDa (non-glycosylated)
packaging
pkg of 5 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
-10 to -25°C
Quality Level
Gene Information
human ... TGFB1(7040)
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General description
The TGFB1 gene is mapped to human chromosome 19q13.2 and codes for a 25 kDa peptide. TGF-β1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer.
Application
Transforming Growth Factor-β1 (TGF-β) human has been used :
- to study the expression of PDGFRB (Platelet derived growth factor receptor β) in association with transforming growth factor-β signaling.
- to stimulate lung fibrosis in human fetal lung fibroblast cell line in order to further measure the in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device.
- to study the effect of TGF-β on HTRA1 (high-temperature requirement A1)-affected Wnt target genes.
Biochem/physiol Actions
TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non-neoplastic tissues release transforming growth factors and essentially all cells possess a specific TGF-β1 receptor. In general, cells of mesenchymal origin appear to be stimulated by TGF-β1; whereas, hepatocytes, T and B lymphocytes, keratinocytes, and many epithelial cells are inhibited by the peptide. TGF-β1 interacts with Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Fibroblast Growth Factor (FGF), and T Cell Growth Factor (T-CGF) either by enhancing or antagonizing their characteristic actions. TGF-β1 plays a fundamental role in tissue growth and differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation, and immune cell function. TGF-β1 is associated with the developmental and metastasis of colorectal cancer.
Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.
Physical form
Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5
Analysis Note
The activity was determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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High-Temperature Requirement A1 (Htra1) - A Novel Regulator of Canonical Wnt Signaling
Globus O, et al.
Scientific Reports, 1-12 (2017)
Interplay between TGF-? signaling and receptor tyrosine kinases in tumor development.
Shi Q and Chen YG
Science China: Life Sciences, 60(10), 1133-1114 (2017)
TGF β signalling and its role in tumour pathogenesis
Kaminska B, et al.
Acta Biochimica Polonica, 52, 329-337 (2005)
Role of TGF-? in metastatic colon cancer: it is finally time for targeted therapy.
Villalba M, et al.
Cell and Tissue Research, 370(1), 29-39 (2017)
The transforming growth factor-?1 (TGFB1) gene is associated with chronic obstructive pulmonary disease (COPD)
Celedon JC, et al.
Human Molecular Genetics, 13, 1649-1656 (2004)
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| H8541-5UG | 04061833810712 |
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