M8787
Magnesium chloride solution
PCR Reagent, 25 mM MgCI2 solution for PCR
Synonym(s):
PCR optimization
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About This Item
Linear Formula:
MgCl2
CAS Number:
Molecular Weight:
95.21
NACRES:
NA.52
PubChem Substance ID:
UNSPSC Code:
12352302
MDL number:
Form:
liquid
form
liquid
packaging
vial of 1.5 mL
concentration
25 mM±1 mM
technique(s)
PCR: suitable
color
colorless
application(s)
agriculture
diagnostic assay manufacturing
foreign activity
DNase, RNase, none detected
storage temp.
2-8°C
SMILES string
Cl[Mg]Cl
InChI
1S/2ClH.Mg/h2*1H;/q;;+2/p-2
InChI key
TWRXJAOTZQYOKJ-UHFFFAOYSA-L
General description
Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.
Application
Magnesium chloride solution has been used as a component of the:
- amplification mixture for polymerase chain reaction (PCR)
- amplification mixture in an inter simple sequence repeats (ISSR)-PCR
- reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)
- PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA
- reaction mix for RT-PCR to assess the gene expression
Suitable for optimization of polymerase chain reactions.
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Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Massimiliano Bergallo et al.
American journal of perinatology, 36(10), 1060-1065 (2018-12-01)
Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and
Kadri Õunap et al.
PloS one, 10(7), e0133841-e0133841 (2015-07-28)
The human WBSCR22 protein is a 18S rRNA methyltransferase involved in pre-rRNA processing and ribosome 40S subunit biogenesis. Recent studies have shown that the protein function in ribosome synthesis is independent of its enzymatic activity. In this work, we have
Manal A Farg et al.
Human molecular genetics, 23(13), 3579-3595 (2014-02-20)
Intronic expansion of a hexanucleotide GGGGCC repeat in the chromosome 9 open reading frame 72 (C9ORF72) gene is the major cause of familial amyotrophic lateral sclerosis (ALS) and frontotemporal dementia. However, the cellular function of the C9ORF72 protein remains unknown.