M8883
4-Methylumbelliferyl phosphate
phosphatase substrate, fluorogenic, ≥98% (HPLC), powder
Synonym(s):
4-Methylumbelliferyl-phosphoric acid
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About This Item
Empirical Formula (Hill Notation):
C10H9O6P
CAS Number:
Molecular Weight:
256.15
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
222-137-7
MDL number:
Beilstein/REAXYS Number:
1687229
Product Name
4-Methylumbelliferyl phosphate, phosphatase substrate
Quality Level
assay
≥98% (HPLC)
form
powder
solubility
water: 25 mg/mL, clear to very slightly hazy, colorless
fluorescence
λex 319 nm; λem 384 nm (pH 9.1), λex 360 nm; λem 449 nm (Reaction product)
storage temp.
−20°C
SMILES string
CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12
InChI
1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
InChI key
BCHIXGBGRHLSBE-UHFFFAOYSA-N
General description
Fluorogenic substrate for phosphatases.
Application
4-Methylumbelliferyl phosphate has been used as a substrate for alkaline phosphatase in the peptide-binding assay. It has also been used as a fluorogenic substrate in enzyme-linked immunosorbent assay (ELISA).
Biochem/physiol Actions
4-Methylumbelliferyl phosphate serves as a fluorogenic substrate for calmodulin-dependent protein phosphatase and in kinetic studies of alkaline phosphatase. It is a substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. 4-Methylumbelliferyl phosphate is seven times more sensitive than phenolphthalein monophosphate and 8-13 times more sensitive than with p-nitrophenyl phosphate when used in enzyme immunoassays for the detection of antibodies to human immunodeficiency viruses.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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