P8412
o-Phenylenediamine dihydrochloride
chromogenic, tablet
Synonym(s):
1,2-Phenylenediamine tablet, OPD, OPD Tablet
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About This Item
Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
Molecular Weight:
181.06
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
3912045
Product Name
o-Phenylenediamine dihydrochloride, tablet, 30 mg substrate per tablet
Quality Level
form
tablet
mp
258 °C (dec.) (lit.)
solubility
water: 1 tablet/10 mL, clear, colorless
storage temp.
2-8°C
SMILES string
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
General description
o-Phenylenediamine dihydrochloride is a binucleophile and its condensation with α,β-unsaturated carbonyl compounds and aldehydes yields benzimidazoles.
o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.
Application
o-Phenylenediamine dihydrochloride has been used as a substrate for horse radish peroxidase in detecting:
- human immunoglobulin
- CTLA4Ig in serum samples
- albumin in culture medium
Preparation Note
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
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Warning
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
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Related Content
Acetic acid-promoted condensation of o-phenylenediamine with aldehydes into 2-aryl-1-(arylmethyl)-1H-benzimidazoles under microwave irradiation
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The reaction of o-phenylenediamine with alpha, beta-unsaturated carbonyl compounds
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