モノクロナール抗β-チューブリン マウス宿主抗体

β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. β-tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins.Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)).

Strongylocentrotus purpuratus(ウニ)精子軸糸由来のサルコシル耐性リボン。

Monoclonal Anti-β-Tubulin antibody produced in mouse has been used in western blotting and fluorescence immunocytochemical staining.

抗体は、自由生活性土壌線虫C. elegans.のβ-チュ-ブリンの代表的な2種類のアイソフォ-ムと1種類の少数アイソフォ-ムを認識します。また、Strongylocentrotus purpuratus精子軸糸由来のサルコシル耐性リボンや幼生ホヤ(Ciona intestinalis)の神経軸索をはじめ、ニワトリや哺乳動物、培養ヒト繊維芽細胞、ウシおよびラット脳組織のチュ-ブリンとも結合します。線虫C.elegansサンプルの場合、抗体は等電点電気泳動ゲルで分離されたチュ-ブリンには結合しますが、SDSやβ-メルカプトエタノ-ル存在下で熱変性したチュ-ブリンは認識しません。哺乳類サンプルのイムノブロッティングでは、熱変性や還元処理しても反応します。

Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

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