05-1075
Anti-FOXO1 Antibody, clone 2H8.2
clone 2H8.2, from mouse
Synonym(s):
Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, forkhead box O1, forkhead box O1A (rhabdomyosarcoma), forkhead homolog in rhabdomyosarcoma
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-FOXO1 Antibody, clone 2H8.2, clone 2H8.2, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2H8.2, monoclonal
species reactivity
rat, human, mouse
packaging
antibody small pack of 25 μg
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FOXO1(2308)
mouse ... Foxo1(56458)
Related Categories
Application
Research Sub Category
Transcription Factors
Transcription Factors
Immunocytochemistry: A 1:500 dilution of a previous lot was used.
Immunocytochemistry: Representative lot data.
A 1:500 dilution of a previous lot was used.
Immunohistochemistry (Paraffin): Representative lot data.
Optimal Staining With Epitope Retrieval: Ductal Carcinoma In Situ.
Immunocytochemistry: Representative lot data.
A 1:500 dilution of a previous lot was used.
Immunohistochemistry (Paraffin): Representative lot data.
Optimal Staining With Epitope Retrieval: Ductal Carcinoma In Situ.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Use Anti-FOXO1 Antibody, clone 2H8.2 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXO1 also known as Forkhead box protein O1A, Forkhead in rhabdomyosarcoma.
Analysis Note
Control
HEK293 cell lysate
HEK293 cell lysate
Evaluated by western blot on HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected FOXO1 in HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected FOXO1 in HEK293 cell lysate.
Biochem/physiol Actions
This antibody detects FOXO1.
FOXO1 shuttles between the nucleus and the cytosol.
FOXO1 shuttles between the nucleus and the cytosol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. (Gross, D.N., 2009).
~78-82 kDa
Immunogen
GST-tagged recombinant protein corresponding to full length FOXO1.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1005
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Dongjie Liu et al.
Oncology letters, 17(1), 450-455 (2019-01-19)
According to global cancer statistics in 2012, lung cancer (LC) was the most frequently diagnosed cancer and the leading cause of cancer-associated mortality among males worldwide. Owing to the limited therapeutic approaches available, novel methods for treating LC are required.
Kevin Dessalle et al.
PloS one, 7(11), e50878-e50878 (2012-12-12)
SREBP-1 are ubiquitously expressed transcription factors, strongly expressed in lipogenic tissues where they regulate several metabolic processes like fatty acid synthesis. In skeletal muscle, SREBP-1 proteins regulate the expression of hundreds of genes, and we previously showed that their overexpression
Jitendra Kumar et al.
Biological trace element research, 196(2), 552-564 (2019-12-13)
The excess fluoride intake has been shown to adversely affect male reproductive health. The aim of the present study was to investigate the key mechanism underlying fluoride-induced testicular dysfunction and the role of melatonin as a modulator of testicular metabolic
Ping Yang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(4), 5658-5672 (2020-02-27)
A contradictory role of CD36 in insulin resistance was found to be related to the nutrient state. Here, we examined that the physiological functions of CD36 in insulin signal transduction in mice fed a low-fat diet. CD36 deficiency led to
Kanako Tamura et al.
PloS one, 10(5), e0126003-e0126003 (2015-05-06)
Glucagon-like peptide-1 (GLP-1) receptor agonists potentiate glucose-induced insulin secretion. In addition, they have been reported to increase pancreatic beta cell mass in diabetic rodents. However, the precise mode of action of GLP-1 receptor agonists still needs to be elucidated. Here
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-1075 | 04053252737688 |
| 05-1075-25UG | 04054839350153 |
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