05-678
Anti-ubiquityl-Histone H2A Antibody, clone E6C5
clone E6C5, Upstate®, from mouse
Synonym(s):
H2AUb, Histone H2A (ubiquityl), H2A histone family, member R, histone 1, H2aa, histone H2A, histone cluster 1, H2aa, H2AK119Ub1
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-ubiquityl-Histone H2A Antibody, clone E6C5, clone E6C5, Upstate®, from mouse
isotype
IgM
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
E6C5, monoclonal
species reactivity
amphibian, mouse, human, frog, rat, monkey
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
frog ... H2Ac1(594915)
human ... H2AC1(221613)
mouse ... H2Ac1(319163)
rat ... H2Ac1(24828)
Analysis Note
Control
Acid extracts of HeLa cells.
Acid extracts of HeLa cells.
Routinely evaluated by western blot on H2A in acid extracts from HeLa and 10T1/2 cells.
Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected ubiquityl-Histone H2A in acid extracts from HeLa and 10T1/2 cells.
Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected ubiquityl-Histone H2A in acid extracts from HeLa and 10T1/2 cells.
Application
Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect ubiquityl-Histone H2A in cells fixed with either 3% formaldehyde/0.1% Triton X-100 or methanol (Vassilev, A. 1995).
Chromatin Immunoprecipitation:
Reported by an independent laboratory (Wang, H. 2004).
This antibody has been reported by an independent laboratory to detect ubiquityl-Histone H2A in cells fixed with either 3% formaldehyde/0.1% Triton X-100 or methanol (Vassilev, A. 1995).
Chromatin Immunoprecipitation:
Reported by an independent laboratory (Wang, H. 2004).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-ubiquityl-Histone H2A Antibody, clone E6C5 (Mouse Monoclonal Antibody) has been published and validated for use in ChIP, ICC, WB to detect ubiquityl-Histone H2A also known as H2AUb, Histone H2A (ubiquityl), histone H2A.
Biochem/physiol Actions
Does not cross-react with army worm.
Other species not tested.
This antibody recognizes and is specific for monoubiquityl-Histone H2A (Lys119), Mr ~25 kDa.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
25 kDa
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones are modified post-translationally by acetylation, phosphorylation, methylation, and ubiquitination and these modifications regulate DNA transcription, repair, recombination, and replication. Ubiquitylation usually targets the substrate for degradation, although histones H2A and H2B are actually stabilized by a single ubiquitin conjugation. Histone ubiquitination has been correlated with DNA repair and transcription, cellular differentiation, cell cycle regulation, spermatogenesis, protein trafficking, and response to stress. Histone H2A is monoubiquitinated at Lys119 by the PRC-1L complex (Polycomb repressive complex 1-like), which includes Ring1, Ring2, Bmi1 and HPH2. Ubiquitinated H2A normally represents about 15% of H2A, but this value can be as high as 50% in active chromatin. Ubiquitinyl histone H2A has also been associated with transcriptional silencing of large chromatin regions and linked to Polycomb silencing so the function of ubiquitinated H2A remains undefined.
Immunogen
AMA (human epithelial amnion) cell residual nuclear pellet portions.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified mouse monoclonal IgM in buffer containing PBS with 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC.
Preparation Note
Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Wei-Ting Liao et al.
Carcinogenesis, 30(6), 1064-1072 (2009-04-21)
Bowen's disease (BD), a carcinoma in situ of the skin, has been identified as an early lesion in arsenic carcinogenesis. Patients with arsenic-induced Bowen's disease (As-BD) showed both cutaneous and systemic immune dysfunctions. We set out to evaluate the interactions
Yanlai Lai et al.
Archives of biochemistry and biophysics, 518(2), 103-110 (2012-01-12)
Inactivation of the von Hippel-Lindau (VHL) tumor suppressor is associated with renal carcinoma, hemangioblastoma and pheochromocytoma. The VHL protein is a component of a ubiquitin ligase complex that ubiquitinates and degrades hypoxia inducible factor-α (HIF-α). Degradation of HIF-α by VHL
MOF and H4 K16 acetylation play important roles in DNA damage repair by modulating recruitment of DNA damage repair protein Mdc1.
Li, X; Corsa, CA; Pan, PW; Wu, L; Ferguson, D; Yu, X; Min, J; Dou, Y
Molecular and cellular biology null
Nickel compounds induce histone ubiquitination by inhibiting histone deubiquitinating enzyme activity.
Qingdong Ke, Thomas P Ellen, Max Costa, Qingdong Ke, Thomas P Ellen, Max Costa
Toxicology and Applied Pharmacology null
Qin Li et al.
Carcinogenesis, 30(7), 1243-1251 (2009-04-21)
In the present study, we examined the effects of CoCl(2) on multiple histone modifications at the global level. We found that in both human lung carcinoma A549 cells and human bronchial epithelial Beas-2B cells, exposure to CoCl(2) (>/=200 muM) for
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-678 | 04053252510571 |
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service