07-329
Anti-acetyl-Histone H4 (Lys16) Antibody
Upstate®, from rabbit
Synonym(s):
Histone H4 Acetylated on Lysine 16, H4K16Ac, Histone H4 (acetyl K16), H4 histone family, member A, H4a
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-acetyl-Histone H4 (Lys16) Antibody, Upstate®, from rabbit
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
dot blot: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
acetylation (Lys16)
Quality Level
Gene Information
human ... H4C1(8359)
mouse ... H4C1(326619)
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Analysis Note
Evaluated by Western Blotting in lysate from Sodium Butyrate treated HeLa cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Acetyl-Histone H4 (Lys16) in lysates from Sodium Butyrate treated HeLa cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Acetyl-Histone H4 (Lys16) in lysates from Sodium Butyrate treated HeLa cells.
Application
Anti-acetyl-Histone H4 (Lys16) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H4 (Lys16) also known as H4K16Ac, Histone H4 (acetyl K16 & has been published and validated in ChIP, WB, Mplex, PIA, DB, ChIP-seq.
Chromatin Immunoprecipitation Analysis: 1 ug from a representative lot immunoprecipitated Acetyl-Histone H4 (Lys16) in HeLa chromatin.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in HeLa, A431, HUVEC, and NIH/3T3 cell lines.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in
an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in HeLa, A431, HUVEC, and NIH/3T3 cell lines.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in
an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Biochem/physiol Actions
Broad species cross-reactivity is expected.
This rabbit polyclonal antibody detects human Histone H4 acetylated on Lysine 16.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
10 kDa
Histone H4, Acetylated on Lysine 16 (UniProt: P62805; also known as H4K16Ac, Histone H4 (acetyl K16), H4 histone family, member A, H4a) is encoded by the HIST1H4A (also known as H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, HIST1H4I, H4/N, HIST1H4B) gene (Gene ID: 121504, 554313, 8294, 8359, 8360, 8361, 8362, 8363, 8364, 8365, 8366, 8367, 8368, 8370) in human. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 features a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone H4 are known to be acetylated on Lysine 5, 8, 12, and 16. Acetylations are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Hyper-acetylation of the histone tails is shown to neutralize positive charge and weakens histone-DNA and nucleosome-nucleosome interactions that destabilizes chromatin structure and increases the accessibility of DNA to various DNA-binding proteins. Chromosomal aberrations involving histone H4 is a cause of B-cell non-Hodgkin lymphomas.
Immunogen
Epitope: N-terminus (Lys 16)
KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H4 acetylated on Lysine 16.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Preparation Note
Stable for 1 year at 2-8° C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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