07-436
Anti-monomethyl-Histone H3 (Lys4) Antibody
Upstate®, from rabbit
Synonym(s):
H3K4me1, Histone H3 (mono methyl K4), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-monomethyl-Histone H3 (Lys4) Antibody, Upstate®, from rabbit
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, vertebrates
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
inhibition assay: suitable (peptide)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
monomethylation (Lys4)
Quality Level
Gene Information
human ... H3C1(8350)
Analysis Note
Control
HeLa cell extract
HeLa cell extract
Routinely evaluated by Western Blot in acid extracted proteins from HeLa cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected methylated histone H3 in acid extracted proteins from HeLa cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected methylated histone H3 in acid extracted proteins from HeLa cells.
Application
Anti-monomethyl-Histone H3 (Lys4) Antibody is a rabbit polyclonal antibody for detection of monomethyl-Histone H3 (Lys4) also known as H3K4me1, Histone H3 (mono methyl K4) & has been validated in ICC, IF, PIA, WB, ChIP-seq, DB.
Immunocytochemistry:
Reported by an independent laboratory.
Peptide Inhibition:
Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. (see figure, lane 3). No competition was observed with peptides containing dimethyl-lysine 4, trimethyl-lysine 4, mono-methyllysine 4 or monomethyl-lysine 9 or 27.
Reported by an independent laboratory.
Peptide Inhibition:
Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. (see figure, lane 3). No competition was observed with peptides containing dimethyl-lysine 4, trimethyl-lysine 4, mono-methyllysine 4 or monomethyl-lysine 9 or 27.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Biochem/physiol Actions
Broad species to cross-reactivity is expected.
Recognizes monomethyl-histone H3 (Lys4), Mr 17kDa.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
17 kDa
Immunogen
Epitope: Lys4
KLH-conjugated, synthetic peptide containing the sequence ART[meK]…, in which meK corresponds to monomethyl-lysine at residue 4 of human histone H3.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.
Preparation Note
Stable for 1 year at 2 to 8°C from date of receipt.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Th2 LCR is essential for regulation of Th2 cytokine genes and for pathogenesis of allergic asthma.
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Min Gyu Lee et al.
Methods (San Diego, Calif.), 40(4), 327-330 (2006-11-15)
Histone methylation is involved in the regulation of many cellular processes. In the past 2 years, several histone demethylases including BHC110/LSD1 have been characterized. BHC110, the first known histone lysine demethylase, removes methyl groups from methylated histone H3 lysine 4
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 07-436 | 04053252364976 |
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