126575
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free
Synonym(s):
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free
description
Merck USA index - 14, 8468
assay
≥95% (cellulose acetate electrophoresis)
form
powder
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤0.02% fatty acids
≤3% ash
≤5% moisture
color
white to light yellow
light yellow to light brown
shipped in
ambient
storage temp.
2-8°C
Related Categories
Application
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free has been used:
- as a component of: blocking buffer immunoelectron microscopy (IEM) for various cells
- mitochondrial isolation buffer (MIB) and mitochondria assay solution (MAS) to trap free fatty acids released during adipose tissue homogenization
- blocking buffer for wholemount staining of the esophageal epithelium (EE) in immunofluorescence
Biochem/physiol Actions
Bovine Serum Albumin (BSA), the most vital component of cell culture media, aids in embryonic stem cells (hESC) differentiation. It aids the transportation of drugs, hormones, and fatty acids. BSA is also used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
Disclaimer
Toxicity: Standard Handling (A)
General description
Albumin, Bovine Serum, (BSA) Fraction V, Fatty Acid-Free is useful for immunodiagnostic assays. It is designed for use in serological testing, radioimmunoassay, and hormone response studies. It is also applicable in binding and transport studies. BSA is prepared by cold ethanol extraction (Cohn method) with a pH: 6.8-7.4.
Bovine serum albumin (BSA) is a non-glycosylated globular, α-helical protein belonging to the serum albumin family. It consists of three domains with two sub-domains each and has 17-disulfide bonds.
Preparation Note
Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.
May take up to 24 h to fully dissolve.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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T Akao et al.
Journal of biochemistry, 99(5), 1425-1431 (1986-05-01)
Ruminococcus sp. PO1-3 obtained from human intestinal flora is able to reduce dehydrocholate as well as 3-ketoglycyrrhetinate. From this bacterium dehydrocholate- and 3-ketoglycyrrhetinate-reducing activities were purified one thousand-fold together with 3-ketocholanate-reducing and 3-beta-hydroxyglycyrrhetinate (glycyrrhetic acid) oxidizing activities by means of
M Prats
European journal of biochemistry, 75(2), 619-625 (1977-05-16)
The L-(+)-Lactate:cytochrome c oxidoreductase or cytochrome b2 from the yeasts Saccharomyces cerevisiae and Hansenula anomala were partially hydrolysed in various concentrations of trypsin. Conditions were found which allowed the isolation from the Hansenula enzyme of a 140 000 +/- 10
Alexandros Tzatsos et al.
Molecular and cellular biology, 26(1), 63-76 (2005-12-16)
Nutritional excess and/or obesity represent well-known predisposition factors for the development of non-insulin-dependent diabetes mellitus (NIDDM). However, molecular links between obesity and NIDDM are only beginning to emerge. Here, we demonstrate that nutrients suppress phosphatidylinositol 3 (PI3)-kinase/Akt signaling via Raptor-dependent
Saeko Akiyama et al.
Stem cell research & therapy, 13(1), 104-104 (2022-03-14)
The liver plays an important role in various metabolic processes, including protein synthesis, lipid and drug metabolisms and detoxifications. Primary culture of hepatocytes is used for the understanding of liver physiology as well as for the drug development. Hepatocytes are
Erica L Scheller et al.
Nature communications, 6, 7808-7808 (2015-08-08)
Marrow adipose tissue (MAT) accumulates in diverse clinical conditions but remains poorly understood. Here we show region-specific variation in MAT adipocyte development, regulation, size, lipid composition, gene expression and genetic determinants. Early MAT formation in mice is conserved, whereas later
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