164545
7-Amino-4-methylcoumarin
≥98% (HPLC), solid
Synonym(s):
7-Amino-4-methylcoumarin, AMC, Coumarin 120
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Empirical Formula (Hill Notation):
C10H9NO2
CAS Number:
Molecular Weight:
175.18
UNSPSC Code:
12352200
NACRES:
NA.21
MDL number:
Product Name
7-Amino-4-methylcoumarin, Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity.
Quality Segment
assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
yellow
solubility
DMSO: 10 mg/mL, acetone: 10 mg/mL, DMF: soluble
fluorescence
λex 365-380 nm, λem 430-460 nm
shipped in
ambient
storage temp.
2-8°C
SMILES string
Nc1cc2c(cc1)C(=CC(=O)O2)C
InChI
1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3
InChI key
GLNDAGDHSLMOKX-UHFFFAOYSA-N
General description
Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity. Useful as a reference standard in enzyme assays.
Reagent used to prepare fluorogenic 7-amino-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzymes (excitation maximum: ~365-380 nm; emission maximum: ~430-460 nm). Also useful as a reference standard in enzyme assays.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Detection of proteolytic enzyme activity
Detection of proteolytic enzyme activity
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). DMF and DMSO stock solutions are stable for up to 6 months at -20°C. Acetone stock solutions are stable for up to 1 week at 4°C or -20°C.
Other Notes
Kanaoka, Y., et al. 1982. Chem. Pharm. Bull. 30, 1485.
Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.
Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
Still not finding the right product?
Explore all of our products under 7-Amino-4-methylcoumarin
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.