MAB079-1
Anti-S-100 Protein Antibody, clone 15E2E2
clone 15E2E2, Chemicon®, from mouse
Synonym(s):
Calcium Binding Protein A1/B1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-S-100 Protein Antibody, clone 15E2E2, clone 15E2E2, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
15E2E2, monoclonal
species reactivity
bovine, rat, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... S100A1(6271)
Analysis Note
Control
Positive Control Tissue: Melanoma
Positive Control Tissue: Melanoma
Evaluated by Western Blot on PC12 lysates
Western Blot Analysis:
1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate.
Western Blot Analysis:
1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate.
Application
Research Sub Category
Muscle Physiology
Muscle Physiology
Anti-S-100 Protein Antibody, clone 15E2E2 is an antibody against S-100 Protein for use in IH, IH(P), IP & WB.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.
Immunohistochemistry:
A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used in IP.
Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.
Immunohistochemistry:
A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.
Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Metabolism
Biochem/physiol Actions
This antibody recognizes ~22 kDa (non-reduced) or 11 kDa (reduced) of S100. It reacts with human, rat, mouse, and cow (bovine). Other species are not tested.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
S-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.
Immunogen
Epitope: Recognizes both alpha & beta S100
Purified bovine S-100 protein
Other Notes
Concentration: 0.5 mg/mL
Physical form
Format: Purified
The mouse IgG is purified by protein A-affinity chromatography according to isotyping.
This affinity purified antibody is supplied in sterile phosphate buffered saline (pH 7.2) containing antibody stabilizer. Contains 0.01% sodium azide.
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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RAGE-TXNIP axis is required for S100B-promoted Schwann cell migration, fibronectin expression and cytokine secretion.
Sbai, O; Devi, TS; Melone, MA; Feron, F; Khrestchatisky, M; Singh, LP; Perrone, L
Journal of Cell Science null
Fanni Dóra et al.
International journal of molecular sciences, 23(13) (2022-07-10)
The default mode network (DMN) plays an outstanding role in psychiatric disorders. Still, gene expressional changes in its major component, the dorsomedial prefrontal cortex (DMPFC), have not been characterized. We used RNA sequencing in postmortem DMPFC samples to investigate suicide
Immunohistochemical visualization of neurons and specific glial cells for stereological application in the porcine neocortex.
Lise Lyck, Jacob Jelsing, Pia S?ndergaard Jensen, Kate Lykke Lambertsen, Bente Pakkenberg et al.
Journal of Neuroscience Methods null
Giant schwannoma of the cheek--a comprehensive histological and immunohistochemical description of a rare tumour.
Mariusz Gajda, Olaf Zagolski, Agnieszka Jasztal, Grzegorz J Lis, Dariusz Adamek et al.
Polish Journal of Pathology : Official Journal of the Polish Society of Pathologists null
Jonathan D Verrier et al.
The Journal of pharmacology and experimental therapeutics, 354(2), 175-183 (2015-05-23)
The 3',5'-cAMP-adenosine pathway (3',5'-cAMP→5'-AMP→adenosine) and the 2',3'-cAMP-adenosine pathway (2',3'-cAMP→2'-AMP/3'-AMP→adenosine) are active in the brain. Oligodendrocytes participate in the brain 2',3'-cAMP-adenosine pathway via their robust expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase; converts 2',3'-cAMP to 2'-AMP). Because Schwann cells also express CNPase
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