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Merck

MAB3120

Anti-Cre Recombinase Antibody, clone 2D8

ascites fluid, clone 2D8, Chemicon®

Synonym(s):

Anti-Cre Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Cre Recombinase Antibody, clone 2D8, ascites fluid, clone 2D8, Chemicon®

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2D8, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

P06956

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

Escherichia coli ... Cre(2777477)

Analysis Note

Control
HeLa Nuclear Extract

Application

Anti-Cre Recombinase Antibody, clone 2D8 is a well characterized monoclonal antibody. Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Western Blot (natural and recombinant protein):
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.

Immunofluorescence:
A previous lot of this antibody was used on IF.

Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.

ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Cre recombinase. The epitope is located between aa 77 and 343.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

38 kDa
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. Anti-Cre Recombinase Antibody can be used to determine the success of gene manipulations in transgenic animals.

Immunogen

Cre recombinase (aa 77 to 343) fusion protein

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Physical form

Ascites fluid. Contains no preservative
Unpurified

Preparation Note

Stable for 1 year at -20ºC from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Murine cell lines derived from Pten null prostate cancer show the critical role of PTEN in hormone refractory prostate cancer development.
Jiao, J; Wang, S; Qiao, R; Vivanco, I; Watson, PA; Sawyers, CL; Wu, H
Cancer Research null
Hong-Xiang Liu et al.
Developmental biology, 368(2), 294-303 (2012-06-05)
The epithelium of mammalian tongue hosts most of the taste buds that transduce gustatory stimuli into neural signals. In the field of taste biology, taste bud cells have been described as arising from "local epithelium", in distinction from many other
Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration.
Judy Grover, Peter J Roughley
Matrix Biology null
Axon position within the corpus callosum determines contralateral cortical projection.
Zhou, J; Wen, Y; She, L; Sui, YN; Liu, L; Richards, LJ; Poo, MM
Proceedings of the National Academy of Sciences of the USA null
Quality control of astrocyte-directed Cre transgenic mice: the benefits of a direct link between loss of gene expression and reporter activation.
Robert Pascal Requardt, Lech Kaczmarczyk, Pavel Dublin, Anke Wallraff-Beck et al.
Glia null
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