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MABE952

Anti-Histone H3.1 Antibody

Name: Anti-Histone H3.1 Antibody
Brand: MM

rat monoclonal, 1D4F2

Synonym(s):

Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

Clone:

1D4F2, monoclonal

Species reactivity:

human, mouse

Application:

ChIP, ICC, IP, WB

Citations:

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Properties

Product Name

Anti-Histone H3.1 Antibody, clone 1D4F2, clone 1D4F2, 1 mg/mL, from rat

biological source

rat

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1D4F2, monoclonal

species reactivity

human, mouse

concentration

1 mg/mL

technique(s)

ChIP: suitable (ChIP-seq), immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

NP_003520

UniProt accession no.

P68431

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HIST1H3F(8968)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

~17 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human Histone H3.1.

Application

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Histone H3.1 in HeLa cells.

Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected Histone H3.1 in NIH/3T3 cells (Prof. Taro Tachibana, Cell Engineering Corporation.).

Alexa Fluor^™ is a registered trademark of Life Technologies.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Histone H3.1 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.1 (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).

Chromatin Immunoprecipitation Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.1 (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).

This Anti-Histone H3.1 Antibody, clone 1D4F2 is validated for use in western blotting, ICC, IP, ChIP & ChIP-seq for the detection of Histone H3.1.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H3.1 in 10 µg of HeLa acid extract.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c