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About This Item
NACRES:
NA.55
UNSPSC Code:
41106502
Product Name
X-tremeGENE™ HP DNA Transfection Reagent, High-performance polymer reagent for transfecting many cell lines
grade
Molecular Biology
form
liquid (aqueous solution)
usage
1 mL (suitable for 165 transfections)
packaging
pkg of 0.4 mL (06366244001)
pkg of 1.0 mL (06366236001)
pkg of 5 × 1 mL (06366546001)
manufacturer/tradename
Roche
technique(s)
transfection: suitable
storage temp.
−20°C
Quality Level
Related Categories
Analysis Note
Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.
Features and Benefits
X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).
- Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium.
- Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
- Generate physiologically relevant data using a reagent with low cytotoxic effects.
- Increase experimental throughput and enable target evaluation using a simple and consistent protocol.
General description
X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Physical form
Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.
Legal Information
X-tremeGENE is a trademark of Roche
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
334.4 °F
flash_point_c
168 °C
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