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Merck

17779

2,3,5-Triphenyl-tetrazolium chloride solution

suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC

Synonym(s):

TTC solution, TTC supplement, TTC solution, TTC supplement

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About This Item

Empirical Formula (Hill Notation):
C19H15ClN4
CAS Number:
298-96-4
Molecular Weight:
334.80
UNSPSC Code:
41171614
NACRES:
NA.85
PubChem Substance ID:
329751567
MDL number:
MFCD00011963
Beilstein/REAXYS Number:
3923356

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Product Name

2,3,5-Triphenyl-tetrazolium chloride solution, suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC

InChI

1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1

SMILES string

[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4

InChI key

PKDBCJSWQUOKDO-UHFFFAOYSA-M

sterility

sterile (Filtered and Aseptic Handled)

form

liquid

shelf life

limited shelf life, expiry date on the label

mol wt

334.8 g/mol

application(s)

agriculture
environmental
food and beverages
microbiology

storage temp.

2-8°C

suitability

bacteria

Quality Level

100

Related Categories

Application

2,3,5-Triphenyl-tetrazolium chloride (TTC) solution is a redox indicator that can help indicate cellular respiration and identify viable and non-viable cells as well as identify microorganisms capable of reduction of TTC on solid culture media

General description

2,3,5-Triphenyl-tetrazolium chloride (TTC) is a dye largely used for the easy enumeration of microbial colonies in solid culture media. This dye is colorless in the oxidized form and changes to red when reduced by microorganisms, due to the formation of formazan. Live microorganisms, for example, E. coli and other coliforms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells. TTC is highly recommended in testing pasteurized milk because there is a high percentage of microorganisms unable to reduce TTC in pasteurized milk, which cannot be detected by laboratory procedures. Higher concentrations of TTC may have an inhibitory effect on the growth of Gram-positive organisms.

Other Notes

(per vial)
2,3,5-Triphenyl tetrazolium chloride: 0.1 g, distilled water: 10 ml

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Certificates of Analysis (COA)

Lot/Batch Number
BCCJ6782
BCCH0596
BCCG2221
BCCF3302
BCCD4874

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Scientific reports, 9(1), 20017-20017 (2019-12-29)
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Neel R Sodha et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 33(5), 906-913 (2008-03-04)
Ischemia-reperfusion (I/R) injury, often encountered clinically, results in myocardial apoptosis and necrosis. Hydrogen sulfide (H(2)S) is produced endogenously in response to ischemia and thought to be cardioprotective, although its mechanism of action is not fully known. This study investigates cardioprotection
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