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Merck

T9032

Thioglycollate medium

NutriSelect® Plus, according to USP, EP, AOAC®, powder, pkg of 500 g, pkg of 1000 g, for bacteria, suitable for microbiology

Synonym(s):

FTG, Fluid Thioglycolate, Fluid Thioglycollate Medium, Thioglycollate Broth

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Plus
Agency:
AOAC®, EP, according to USP
Form:
powder
Suitability:
bacteria
Application(s):
clinical testing
environmental
food and beverages
sterility testing, microbiology
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Quality Level

agency

AOAC®, EP, according to USP

description

Microbiologically tested

sterility

non-sterile

form

powder

packaging

pkg of 1000 g, pkg of 500 g

manufacturer/tradename

NutriSelect® Plus

application(s)

clinical testing
environmental
food and beverages
sterility testing, microbiology

suitability

bacteria

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Solve 29.8 g in 1 litre distilled water. Autoclave 15 minutes at 121°C.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
AOAC is a registered trademark of AOAC International

Application

Thioglycollate Medium is widely used in clinical testing, environmental monitoring, food and beverage testing, and sterility testing. It supports the growth of both aerobic and anaerobic microorganisms, making it ideal for sterility control in pharmaceutical products and for testing materials containing heavy metals with bacteriostatic effects. The medium is also used to determine an organism′s oxygen requirements and its ability to grow in anaerobic or microaerophilic environments.

Features and Benefits

NutriSelect® Plus is a powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

General description

Thioglycollate Medium is a powdered broth medium designed for the cultivation of both aerobic and anaerobic microorganisms. The medium contains dextrose, tryptone, yeast extract, and L-cystine providing essential growth factors for bacterial proliferation. Sodium thioglycollate and L-cystine serve as reducing agents that prevent the accumulation of lethal peroxides, enabling the growth of Clostridium even under aerobic conditions. Resazurin acts as an oxidation-reduction indicator, appearing pink when oxidized and colorless when reduced. The minimal amount of agar in the medium supports the growth of both aerobes and anaerobes, regardless of the presence of sodium thioglycollate. Sodium thioglycollate also neutralizes the toxic effects of mercurial preservatives and peroxides, promoting anaerobiosis and making the medium suitable for testing materials containing heavy metals. Any increase in oxygen content is indicated by a color change in resazurin from colorless to pink. The small agar content helps maintain a low redox potential, stabilizing the medium.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Justin T Avery et al.
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Elaina M Melton et al.
The Journal of biological chemistry, 294(43), 15836-15849 (2019-09-10)
Cholesterol esters are a key ingredient of foamy cells in atherosclerotic lesions; their formation is catalyzed by two enzymes: acyl-CoA:cholesterol acyltransferases (ACATs; also called sterol O-acyltransferases, or SOATs) ACAT1 and ACAT2. ACAT1 is present in all body cells and is
Micely D R Hermida et al.
BMC research notes, 10(1), 695-695 (2017-12-07)
In this study, we investigate the diversity and modulation of leukocyte populations represented in the gates defined by size and granularity at different time points of thioglycollate-induced peritonitis in mouse. The inflammatory cells were distributed into four regions (R1-R4) of
Borong Zhou et al.
Nature microbiology, 5(12), 1576-1587 (2020-10-21)
Sepsis is the most common cause of death for patients in intensive care worldwide due to a dysregulated host response to infection. Here, we investigate the role of sequestosome-1 (SQSTM1/p62), an autophagy receptor that functions as a regulator of innate
X Xie et al.
The Journal of biological chemistry, 275(44), 34818-34825 (2000-08-17)
Selectins play a major role in the inflammatory reaction by initiating neutrophil attachment to activated vascular endothelium. Some heparin preparations can interact with L- and P-selectin; however, the determinants required for inhibiting selectin-mediated cell adhesion have not yet been characterized.

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