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X100

Triton™ X-100

laboratory grade

Synonym(s):

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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About This Item

Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
9036-19-5
UNSPSC Code:
12161900
NACRES:
NA.21
PubChem Substance ID:
329830772
MDL number:
MFCD00128254
Beilstein/REAXYS Number:
2315025
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biological source

synthetic

Quality Level

200

grade

laboratory grade

description

non-ionic

form

liquid

mol wt

micellar avg mol wt 80,000, average mol wt 625

aggregation number

100-155

CMC

0.2-0.9 mM (20-25°C)

mp

6  °C (43 °F)

transition temp

cloud point 65 °C, pour point ~7 °C

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

General description

Triton X-100 has hemolytic property and is used for DNA extraction.
Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton™-X has been used:
  • In the permeabilization of cells for immunofluorescence staining
  • As a component of lysis buffer in western blot analysis
  • As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

  • Ideal for Cell Biology and Biochemical research applications
  • Non-ionic nondenaturing detergent
  • Superior wetting agent and emulsifier
  • Highly versatile surfactant

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
For additional information on our range of Biochemicals, please complete this form.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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Danger

Storage Class

10 - Combustible liquids

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

9036-19-5

CAS No.

9036-19-5

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
019K0151
118K0160
118K0095
037K00042
028K0011

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Growth and hydration of Triton X-100 micelles in monovalent alkali salts: a light scattering study
Molina-Bolivar JA, et al.
The Journal of Physical Chemistry B, 106(4), 870-877 (2002)
Knockdown of miR-210 decreases hypoxic glioma stem cells stemness and radioresistance
Yang W, et al.
Experimental Cell Research, 326(1), 22-35 (2003)
alpha -Synuclein Is Degraded by Both Autophagy and the Proteasome
Webb LJ, et al.
The Journal of Biological Chemistry, 278(27), 25009-25013 (2003)
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