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Merck

88417

MTT Formazan

MTT reduction end product, powder

Synonym(s):

1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan

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About This Item

Empirical Formula (Hill Notation):
C18H17N5S
CAS Number:
57360-69-7
Molecular Weight:
335.43
UNSPSC Code:
12352204
PubChem Substance ID:
329769650
NACRES:
NA.83
MDL number:
MFCD00080731

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Product Name

MTT Formazan, MTT reduction end product

SMILES string

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

InChI key

GIFKVQOPAJDJLO-SZVLODPRSA-N

form

powder

solubility

chloroform: 10 mg/mL, clear, dark violet

Quality Level

100

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Application

MTT Formazan has been used in cell viability assay.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Other Notes

Reviews: Tetrazolium salts and formazans

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH1758
BCCG8173
BCCG7693
BCCG5793
BCCG5533

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