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Merck

A1595

Bovine Serum Albumin solution

10% in DPBS, low endotoxin, fatty acid free, suitable for cell culture, sterile-filtered

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
9048-46-8
UNSPSC Code:
12352202
NACRES:
NA.27
MDL number:
MFCD00130384

Key Documents

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Product Name

Bovine Serum Albumin solution, 10% in DPBS, low endotoxin, fatty acid free, suitable for cell culture, sterile-filtered

biological source

bovine

sterility

sterile-filtered

product line

BioReagent

assay

90-120 mg/mL protein basis (biuret)

form

solution

purified by

heat shock fractionation

contains

does not contain stabilizer or preservative

origin

USA origin

concentration

10% in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

≤0.05% fatty acid
≤200 EU/mL endotoxin

pH

7.1-7.7

UniProt accession no.

P02769

storage temp.

2-8°C

Quality Level

300

Gene Information

bovine ... ALB(280717)

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Features and Benefits

  • Fatty acid free / tested to be low in fatty acid content
  • Low endotoxin
  • Sterile-filtered

Preparation Note

Prepared with Heat Shock Fractionated fatty acid-free albumin in DPBS D5652.

Application

Recommended for use as a base for the preparation of defined, fatty acid supplements. Can be loaded with up to 4 moles of fatty acids per mole of albumin.

BSA is a known fatty acid (FA) carrier. Thus in FA-related applications like cell culture, the specific BSA can be important, as related to control over specific fatty acids used to culture cell lines, because different cell lines can be variably sensitive to particular FA;s. Fatty acid-free BSA therefore is useful in cell culture work where the specific fatty acid content must be carefully controlled, so that researchers can control the specific FA′s needed for their particular cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific fatty acids that researchers wish to use in their particular cell culture experiments. Use of fatty-acid-free BSA also addresses concerns about endogenous FA′s potentially in non-fatty acid-free BSA.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ1289
SLCF7734
SLCD5004
SLCB4542
SLBT3623

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